Elisa Su'ega Pusa o AOZ
E uiga i
O lenei pusa e mafai ona faʻaaogaina i suʻesuʻega quantitative ma qualitative o totoe o le AOZ i totonu o manu (moa, povi, puaa, ma isi), susu, meli ma fuamoa.
O le suʻesuʻeina o vailaʻau o le nitrofuran e manaʻomia ona faʻavae i luga o le suʻesuʻeina o metabolites fusifusia o le nitrofuran matua fualaau, lea e aofia ai furazolidone metabolite (AOZ), Furaltadone metabolite (AMOZ), Nitrofurantoin metabolite (AHD) ma Nitrofurazone metabolite (SEM).
Pe a faatusatusa i metotia chromatographic, o la matou pusa o loʻo faʻaalia ai le tele o mea lelei e uiga i le maʻaleʻale, faʻatapulaʻaina o mea, meafaigaluega faʻapitoa ma taimi manaʻomia.
Vaega Vaega
• Microtiter ipu ua ufiufi i antigen, 96 vaieli
• Fofo masani (6 fagu,1ml/fagu)
0ppb,0.025ppb,0.075ppb,0.225ppb,0.675ppb,2.025ppb
• Fa'ata'ita'i fa'atonu : (1ml/fagu)................................................….100ppb
• Enzyme conjugate concentrate 1.5ml.................................................…..pulou mumu
• vaifofo fa'aantibody fa'atumauina 0.8ml……………………………………...… pulou lanu meamata
• Fa'amea A 7ml....................................................................................pulou paepae
• Lafu B7ml………………………………………….....................................................pulou mumu
• Taofi fofo 7ml…………………………………………………….……… pulou samasama
• 20x vaifofo taele 40ml ………………………………………..pulou manino
• 2×fa'ato'a fa'amama vaifofo 60ml……………………..………….pulou lanumoana
• 2-Nitrobenzaldehyde 15.1mg…………………………………………………… pulou uliuli
Ma'ale'ale, sa'o ma sa'o
Ma'ale'ale: 0.025ppb
Fa'atapula'a su'esu'ega……………………………………………0.1ppb
Sa'o:
Aano o manu (muso ma le ate)……………………75±15%
Meli……………………………………………………..90±20%
Fuamoa……………………………………………..…90±20%
Suasusu………………………………………………………………..90±10%
Sa'o:CV o le pusa ELISA e itiiti ifo i le 10%.
Fua Faatatau
Furazolidone metabolite (AOZ)………………………………………………..100%
Furaltadone metabolite (AMOZ)……………………………………………………<0.1%
Nitrofurantoin metabolite (AHD)…………………………………………<0.1%
Nitrofurazone metabolite (SEM)…………………………………………...<0.1%
Furazolidone………………………………………………………………..16.3%
Furaltadone……………………………………………………………………<1%
Nitrofurantoin………………………………………………………….…<1%
Nitrofurazone……………………………………………………………………..<1%