Elisa Test Kit of Aflatoxin B1
Ole tele o tui ole aflatoxins e o'o atu ai ile o'ona tuga (aflatoxicosis) e ono lamatia ai le ola, e masani lava ile fa'aleagaina ole ate.
Aflatoxin B1 o se aflatoxin gaosia e Aspergillus flavus ma A. parasiticus.Ose tino mamana malosi.O lenei malosi fa'ama'i e 'ese'ese i ituaiga ma nisi, e pei o isumu ma manuki, e foliga mai e sili atu ona faigofie nai lo isi.Aflatoxin B1 o se faʻamaʻi masani i meaʻai eseese e aofia ai pinati, fatu fatu, sana, ma isi fatu;fa'apea fo'i mea'ai a manu.Aflatoxin B1 ua manatu o le aflatoxin sili ona oona ma e sili ona aafia i le hepatocellular carcinoma (HCC) i tagata.Ole tele o fa'ata'ita'iga ma metotia au'ili'ili e aofia ai le thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), mass spectrometry, ma enzyme-linked immunosorbent assay (ELISA), faatasi ai ma isi, ua faʻaaogaina e suʻe ai le aflatoxin B1 faʻaleagaina i meaʻai. .E tusa ai ma le Faalapotopotoga o Mea Taumafa ma Faatoaga (FAO), o le maualuga maualuga o le aflatoxin B1 i le lalolagi atoa na lipotia mai i le va o le 1-20 μg / kg i meaʻai, ma le 5-50 μg / kg i meaʻai povi i le 2003.
Fa'amatalaga
1. Elisa Su'ega Pusa mo Aflatoxin B1
2. Pusi.KA07202H-96vaieli
3. Vaega o pusa
● Microtiter plateprecoated ma antigen, 96 vaieli
● Fofo masani ×6 fagu(1ml/fagu)
0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb
● Enzyme conjugate 7ml…………………………………………………………………………………… pulou mumu
● Antibody solution7ml............................................... ...................................................pulou lanu meamata
● Lapisi A 7ml………………………………………………………………………………………pulopa paepae
● Su'ega B 7ml……………………………………………………………………………………..pulou mumu
● Taofi le vaifofo 7ml……….…….……………………………………………………………………..……… pulou samasama
● 20 × fa'amamafa vaifofo fufulu 40ml ………………………………………… pulou manino
● 2 × fa'aputu fa'aputu fofo 50ml …………………………………………………… pulou lanumoana
4.Sensitivity, saʻo ma saʻo
Ma'ale'ale: 0.05ppb
5. Su'esu'ega tapula'a
Fa'ata'ita'iga o le suau'u taumafa................................................. ................................................... ........................0.1ppb
Pinati................................................. ................................................... .......................0.2ppb
Saito................................................. ................................................... ......................0.05ppb
Sa'o
Fa'ata'ita'iga o le suau'u taumafa................................................. ................................................... ....................80±15%
Pinati................................................. ................................................... .....................80±15%
Saito................................................. ................................................... .....................80±15%
Sa'o:Ole fa'asologa ole suiga ole pusa ELISA e itiiti ifo ile 10%.
6. Fua Faatatau
Aflatoxin B1················100%
Aflatoxin B2·······················81 .3%
Aflatoxin G1······················62%
Aflatoxin G2······················· 22.3%