ibicuruzwa

Nitrofurans ihinduranya imbere mu mubiri vuba cyane, kandi metabolite yabo ifatanije nuduce twabaho igihe kirekire, bityo isesengura ryibisigisigi by’ibi biyobyabwenge bizaterwa no kumenya metabolite zabo, harimo metabolite ya furazolidone (AOZ), metabolite ya furaltadone (AMOZ) ), nitrofurantoin metabolite (AHD) na nitrofurazone metabolite (SEM).

Kwinbon Irushanwa Enzyme Immunoassay ibikoresho, izwi kandi nka Elisa kits, ni tekinoroji ya bioassay ishingiye ku ihame rya Enzyme-Ihuza Immunosorbent Assay (ELISA). Ibyiza byayo bigaragarira cyane cyane mubice bikurikira:

 (1) Kwihuta: Ubusanzwe laboratoire ifata LC-MS na LC-MS / MS kugirango ibone metabolite ya nitrofurazone. Nyamara Kwinbon ELISA ikizamini, aho antibody yihariye ikomoka kuri SEM isobanutse neza, yunvikana, kandi yoroshye gukora. Igihe cyo gusuzuma iki gikoresho ni 1.5h gusa, nigikorwa cyiza cyo kubona ibisubizo. Ibi nibyingenzi mugusuzuma byihuse no kugabanya ubukana bwakazi.

(2) Ukuri: Bitewe numwihariko wo hejuru hamwe na sensitivite ya Kwinbon SEM Elisa kit, ibisubizo birasobanutse neza hamwe na marike yo kwibeshya. Ibi bituma ikoreshwa cyane muri laboratoire z’amavuriro n’ibigo by’ubushakashatsi kugira ngo ifashe ubworozi bw’uburobyi n’ibicuruzwa byoherezwa mu mahanga mu gusuzuma no kugenzura ibisigazwa by’imiti y’amatungo ya SEM mu bicuruzwa byo mu mazi.

(3) Umwihariko: Kwinbon SEM Elisa kit ifite umwihariko kandi irashobora kugeragezwa kurwanya antibody yihariye. Umusaraba reaction ya SEM na metabolite yayo ni 100%. Corss reaction yerekana munsi ya 0.1% ya AOZ, AMOZ, AHD, CAP na metabolite yabo, Ifasha kwirinda kwisuzumisha nabi no kutayireka.