China Inkingi Inkingi yo Gutahura Uruganda rwa zearalenone n'abakora

Inkingi ya Immunoafficine yo kumenya amashusho yerekana

Inkingi ya Immunoafficine yo kumenya kwa zearalenone

Ibisobanuro bigufi:

Kwinbon Zearalenone inkingi zikoreshwa muguhuza na HPLC, LC-MS, Ikizamini cya Elisa. Birashobora kuba byinshi kugerageza AFB1 ku binyampeke n'ibikoresho by'ingano, isosi, Ibicuruzwa, inzoga, soya, kugaburira ibikoresho bibisi, nibindi

Ohereza imeri kuri twe Icyerekezo cyo gukoresha

Ibisobanuro birambuye

Ibicuruzwa

Injangwe oya.	Kh00304z
Umutungo	KuriZearalenonekwipimisha
Aho inkomoko	Beijing, Ubushinwa
Izina	Kwinbon
Ingano	Ibizamini 25 kuri buri gasanduku
Icyitegererezo	Grains and grain products, soy sauce, vinegar, sauce products, alcohol, soybeans, rapeseed and vegetable oils, feed raw materials and finished products, etc.
Ububiko	2-30 ℃
Imibereho-Ubuzima	Amezi 12
GUTANGA	Icyumba cyambere

Ibikoresho & reagent bisabwa

Ibikoresho

Reagent

Ibikoresho

---- homogenizer ---- Vortex mixer

---- Icumbi ---- Gupima Cylinder: 10ml, 100ml

---- Umunyarubuga Impapuro / Centrifuge ---- Impirimbanyi zisesengura (Inductance: 0.01g)

---- Umuyoboro wangiritse: 10ml ---- injiza: 20ml

---- Flask Flask: 250ml ---- Rubber piettte

---- Micropipette: 100-1000ul ---- Ikirahure cya 50ml

---- Akayunguruzo ka Microfiber (Whatman, 934-Ah, φ11cm, 1.5um Uruziga)

Reagent

---- methanol (ar)

---- aside acide (ar)

---- Chloride ya Sodium (Nacl, Ar)

---- amazi ya deionized

Ibyiza Byibicuruzwa

Zearalenone (zen), uzwi kandi nka RAL na F-2 MyCotoxin, ni Estrogyenic Metabolite yakozwe. Nubushyuhe kandi buboneka kwisi yose mumibiri y'ibinyampeke, nk'ibigori, sayiri, ingano, umuceri, umukandari, n'umusaka.

Zearalenone ni ubuhe buryo bw'ibanze buhuza na estrogenter estroge batoranijwe, bigatuma ubupfumba, gukuramo inda cyangwa ibindi bibazo, cyane cyane mu ngurube.

Wikipedia irasaba uburyo bukurikira;

Thin Layer chromatografiya,
Enzyme-linked immnunsorbent assay
Umuvumo wa Imyumbati
Umuvumo wa Imyunguriro Imikorere minini yimikorere ya chromatografiya

Kwinbon Inmmunoaffinity Inkingi ni iya gatatu, ikoreshwa rya chromatografiya yo gutandukana, kwezwa cyangwa gusesengura noaralenone. Mubisanzwe inkingi ya Kwinbon ihujwe na HPLC.

Isesengura rya HPLC Isesengura ryibiciro byibiciro ni tekinike yo kumenya. Byombi imbere na guhindura icyiciro cya chromatografiya birakoreshwa. Hindura icyiciro HPLC ni ubukungu, byoroshye gukora, kandi bifite uburozi buke. Uburozi bwinshi burashonga mu byiciro bya Polor hanyuma bitandukana ninkingi za chromatography, zihura nibikenewe gutahura byihuse toxine zamata. UPLC yahujwe buhoro buhoro ishyirwa mu gaciro, hamwe na module yo hejuru hamwe nubunini buto hamwe ningano nini ya chromatografiya, kunoza icyitegererezo cyo gutandukana, kunoza imikorere yo gutandukana, kandi kugera ku kumva kwiyumvisha.

Hamwe n'umwihariko, Kwinbon Zearalenone inkingi zirashobora gufata molekile zigamije muburyo bwera cyane. Kandi inkingi ya Kwinbon igenda yihuta, Eassy gukora. Noneho birahunze kandi nkoresha cyane muburiganya no muri salle ya Mycotoxines.