Ubushinwa Immunoaffinity inkingi zinganda zimenyekanisha Zearalenone nababikora

Immunoaffinity inkingi zo kumenya Zearalenone

Ibisobanuro bigufi:

Inkingi za Kwinbon Zearalenone zikoreshwa muguhuza HPLC, LC-MS, ibikoresho bya ELISA. Irashobora kuba igeragezwa ryinshi AFB1 kubinyampeke n'ibinyampeke, isosi ya soya, vinegere, ibikomoka ku isosi, inzoga, soya, amavuta yo gufata ku ngufu n'imboga, kugaburira ibikoresho fatizo n'ibicuruzwa byarangiye, n'ibindi.

Ohereza imeri kuri twe Amabwiriza yo Gukoresha

Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro ku bicuruzwa

Injangwe no.	KH00304Z
Ibyiza	KuriZearalenoneikizamini
Aho byaturutse	Beijing, Ubushinwa
Izina ry'ikirango	Kwinbon
Ingano yubumwe	Ibizamini 25 kuri buri gasanduku
Icyitegererezo	Ibinyampeke n'ibinyampeke, isosi ya soya, vinegere, ibikomoka ku isosi, inzoga, soya, amavuta y'imboga n'imboga, kugaburira ibikoresho bibisi n'ibicuruzwa byarangiye, n'ibindi.
Ububiko	2-30 ℃
Ubuzima bwa Shelf	Amezi 12
Gutanga	Icyumba cy'ubushyuhe

Ibikoresho & Reagents Birakenewe

Ibikoresho

Reagents

Ibikoresho

---- Homogenizer ---- Vortex ivanga

---- Icupa ry'icyitegererezo ---- Gupima silinderi: 10ml, 100ml

---- Impapuro zungurura impapuro / Centrifuge ---- Impuzandengo yisesengura (inductance: 0.01g)

---- Impamyabumenyi yarangije: 10ml ---- Injiza: 20ml

---- Amashanyarazi ya volumetric: 250ml ---- Rubber pipette

---- Micropipette: 100-1000ul ---- Glass funnel 50ml

---- Akayunguruzo ka Microfibre (Whatman, 934-AH, Φ11cm, 1.5um ruziga)

Reagents

---- Methanol (AR)

---- Acide acike (AR)

---- Sodium chloride (NACL, AR)

---- Amazi ya Deionised

Ibyiza byibicuruzwa

Zearalenone (ZEN), izwi kandi nka RAL na F-2 mycotoxine, ni metabolite ikomeye ya estrogene. Ifite ubushyuhe kandi iboneka ku isi hose mu bihingwa byinshi by’ibinyampeke, nk'ibigori, sayiri, oati, ingano, umuceri, n'amasaka.

Zearalenone nuburozi bwibanze buhuza reseptor ya estrogene, bigatera ubugumba, gukuramo inda cyangwa ibindi bibazo byororoka, cyane cyane mu ngurube.

Wikipedia irasaba uburyo bukurikira bwo gutahura;

Inzira ntoya ya chromatografiya,
Enzyme-ihuza immunosorbent assay
Immunoaffinity inkingi fluorescence
Immunoaffinity inkingi ikora neza ya chromatografiya

Kwinbon Inmmunoaffinity Inkingi nuburyo bwa gatatu, ikoresha chromatografiya yamazi yo gutandukana, kweza cyangwa gusesengura byihariye bya Zearalenone. Mubisanzwe inkingi za Kwinbon zahujwe na HPLC.

HPLC isesengura ryinshi ryuburozi bwa fungal nubuhanga bukuze bwo kumenya. Byombi imbere na revers ya chromatografi irakoreshwa. Guhindura icyiciro HPLC nubukungu, byoroshye gukora, kandi bifite uburozi buke. Uburozi bwinshi burashonga mubice byimikorere ya polar hanyuma bigatandukanywa ninkingi za chromatografiya zidafite inkingi, byujuje ibisabwa kugirango hamenyekane vuba uburozi bwinshi bwibihumyo mubyitegererezo byamata. UPLC ihuriweho na disiketi igenda ikoreshwa buhoro buhoro, hamwe ningutu yumuvuduko mwinshi hamwe nubunini buto hamwe nubunini bwa chromatografiya, bishobora kugabanya icyitegererezo cyo gukora, kunoza imikorere ya chromatografique, kandi bikagera no kubyumva neza.

Hamwe nibisobanuro bihanitse, inkingi za Kwinbon Zearalenone zirashobora gufata molekile zintego muburyo bwiza cyane. Nanone inkingi za Kwinbon zitemba vuba, byoroshye gukora. Noneho birihuta kandi bikoreshwa cyane mubiryo no murima ya mycotoxine decetion.