Ubushinwa Immunoaffinity inkingi ya T-2 mycotoxin detection uruganda nababikora

Immunoaffinity inkingi ya T-2 mycotoxin

Ibisobanuro bigufi:

Kwinbon T-2 inkingi ya mycotoxin ikoreshwa muguhuza HPLC, LC-MS, ELISA ibikoresho byo gupima.Irashobora kuba igeragezwa ryinshi T-2 mycotoxine kubinyampeke n'ibinyampeke, isosi ya soya, vinegere, ibikomoka ku isosi, inzoga, soya, amavuta y'imboga n'imboga, kugaburira ibikoresho fatizo n'ibicuruzwa byarangiye, n'ibindi.

Ohereza imeri kuri twe Amabwiriza yo Gukoresha

Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro ku bicuruzwa

Injangwe no.	KH01301Z
Ibyiza	KuriT-2 mycotoxinikizamini
Aho byaturutse	Beijing, Ubushinwa
Izina ry'ikirango	Kwinbon
Ingano yubumwe	Ibizamini 25 kuri buri gasanduku
Icyitegererezo	Ibinyampeke n'ibinyampeke, isosi ya soya, vinegere, ibicuruzwa by'isosi, inzoga, n'ibindi.
Ububiko	2-30 ℃
Ubuzima bwa Shelf	Amezi 12
Gutanga	Icyumba cy'ubushyuhe

Ibikoresho & Reagents Birakenewe

Ibikoresho

Reagents

Ibikoresho

---- Homogenizer ---- Vortex ivanga

---- Icupa ry'icyitegererezo ---- Gupima silinderi: 10ml, 100ml

---- Impapuro zungurura impapuro / Centrifuge ---- Impuzandengo yisesengura (inductance: 0.01g)

---- Impamyabumenyi yarangije: 10ml ---- Injiza: 20ml

---- Amashanyarazi ya volumetric: 250ml ---- Rubber pipette

---- Micropipette: 100-1000ul ---- Glass funnel 50ml

---- Akayunguruzo ka Microfibre (Whatman, 934-AH, Φ11cm, 1.5um ruziga)

Reagents

---- Methanol (AR)

---- Acide acike (AR)

---- Sodium chloride (NACL, AR)

---- Amazi ya Deionised

Ibyiza byibicuruzwa

T-2 mycotoxine ni ibisanzwe bisanzwe biboneka byumusaruro wa Fusarium spp. fungus kandi ni uburozi kubantu nandi matungo. Imiterere yubuvuzi itera ni alimentary toxic aleukia hamwe nibimenyetso byinshi bifitanye isano ningingo zitandukanye nkuruhu, umwuka, nigifu. Iraboneka muri T-2 mycotoxine ibinyampeke byanduye, ibiryo n'ibiryo.

Kwinbon Inmmunoaffinity Inkingi nuburyo bwa gatatu, ikoresha chromatografiya yamazi yo gutandukana, kweza cyangwa gusesengura byihariye bya T-2 mycotoxine. Mubisanzwe inkingi za Kwinbon zahujwe na HPLC.

HPLC isesengura ryinshi ryuburozi bwa fungal nubuhanga bukuze bwo kumenya. Byombi imbere na revers ya chromatografi irakoreshwa. Guhindura icyiciro HPLC nubukungu, byoroshye gukora, kandi bifite uburozi buke. Uburozi bwinshi burashonga mubice byimikorere ya polar hanyuma bigatandukanywa ninkingi za chromatografiya zidafite inkingi, byujuje ibisabwa kugirango hamenyekane vuba uburozi bwinshi bwibihumyo mubyitegererezo byamata. UPLC ihuriweho na disiketi igenda ikoreshwa buhoro buhoro, hamwe ningutu yumuvuduko mwinshi hamwe nubunini buto hamwe nubunini bwa chromatografiya, bishobora kugabanya icyitegererezo cyo gukora, kunoza imikorere ya chromatografique, kandi bikagera no kubyumva neza.

Hamwe nibisobanuro bihanitse, Kwinbon T-2 inkingi ya mycotoxine irashobora gufata molekile yintego muburyo bwiza cyane. Nanone inkingi za Kwinbon zitemba vuba, byoroshye gukora. Noneho birihuta kandi bikoreshwa cyane mubiryo no murima ya mycotoxine decetion.