Ubushinwa Immunoaffinity inkingi za Ochratoxin Uruganda rukora ibicuruzwa nababikora

Immunoaffinity inkingi ya Ochratoxin Kumenyekanisha Ishusho Yerekanwe

Immunoaffinity inkingi ya Ochratoxin Kumenya

Ibisobanuro bigufi:

Kwinbon Ochratoxin Inkingi zikoreshwa muguhuza na HPLC, LC-MS, ELISA ibikoresho byo gupima. Irashobora kuba igeragezwa ryinshi Ochratoxin A kubinyampeke nibicuruzwa byimbuto, isosi ya soya, vinegere, ibicuruzwa bya sosi, inzoga, Cocoa hamwe nikawa ikaranze, nibindi.

Ohereza imeri kuri twe Amabwiriza yo Gukoresha

Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro ku bicuruzwa

Injangwe no.	KH00404Z
Ibyiza	KuriOchratoxin A. ikizamini
Aho byaturutse	Beijing, Ubushinwa
Izina ry'ikirango	Kwinbon
Ingano yubumwe	Ibizamini 25 kuri buri gasanduku
Icyitegererezo	Gimvura n'ibicuruzwa by'ingano, isosi ya soya, vinegere, ibicuruzwa by'isosi, inzoga, Kakao n'ikawa ikaranze, n'ibindi.
Ububiko	2-30 ℃
Ubuzima bwa Shelf	Amezi 12
Gutanga	Icyumba cy'ubushyuhe

Ibikoresho & Reagents Birakenewe

Ibikoresho

Reagents

Ibikoresho

---- Homogenizer ---- Vortex ivanga

---- Icupa ry'icyitegererezo ---- Gupima silinderi: 10ml, 100ml

---- Impapuro zungurura impapuro / Centrifuge ---- Impuzandengo yisesengura (inductance: 0.01g)

---- Impamyabumenyi yarangije: 10ml ---- Injiza: 20ml

---- Amashanyarazi ya volumetric: 250ml ---- Rubber pipette

---- Micropipette: 100-1000ul ---- Glass funnel 50ml

---- Akayunguruzo ka Microfibre (Whatman, 934-AH, Φ11cm, 1.5um ruziga)

Reagents

---- Methanol (AR)

---- Acide acike (AR)

---- Sodium chloride (NACL, AR)

---- Amazi ya Deionised

Ibyiza byibicuruzwa

Nkuko bizwi na mycotoxine, Ochratoxin A (OTA) ikorwa nubwoko butandukanye bwibihumyo harimo Aspergillus ochraceus, A. karubone, A. niger na Penicillium verrucosum. OTA itera nephrotoxicity n'ibibyimba by'impyiko mu bwoko butandukanye bw'inyamaswa; icyakora, ingaruka zubuzima bwabantu ntizirangwa neza.

Kwinbon Inmmunoaffinity Inkingi nuburyo bwa gatatu, ikoresha chromatografiya yamazi yo gutandukana, kwezwa cyangwa gusesengura byihariye bya Ochratoxin A. Mubisanzwe inkingi za Kwinbon zahujwe na HPLC.

HPLC isesengura ryinshi ryuburozi bwa fungal nubuhanga bukuze bwo kumenya. Byombi imbere na revers ya chromatografi irakoreshwa. Guhindura icyiciro HPLC nubukungu, byoroshye gukora, kandi bifite uburozi buke. Uburozi bwinshi burashonga mubice byimikorere ya polar hanyuma bigatandukanywa ninkingi za chromatografiya zidafite inkingi, byujuje ibisabwa kugirango hamenyekane vuba uburozi bwinshi bwibihumyo mubyitegererezo byamata. UPLC ihuriweho na disiketi igenda ikoreshwa buhoro buhoro, hamwe ningutu yumuvuduko mwinshi hamwe nubunini buto hamwe nubunini bwa chromatografiya, bishobora kugabanya icyitegererezo cyo gukora, kunoza imikorere ya chromatografique, kandi bikagera no kubyumva neza.

Hamwe nibisobanuro bihanitse, Kwinbon Ochratoxin Inkingi irashobora gufata molekile yintego muburyo bwiza cyane. Nanone inkingi za Kwinbon zitemba vuba, byoroshye gukora. Noneho birihuta kandi bikoreshwa cyane mubiryo no murima ya mycotoxine decetion.