Ubushinwa Immunoaffinity inkingi za Aflatoxin Uruganda rwose nababikora

Immunoaffinity inkingi ya Aflatoxin Igishusho Cyuzuye

Immunoaffinity inkingi ya Aflatoxin Yuzuye

Ibisobanuro bigufi:

Inkingi za AFT zikoreshwa muguhuza hamwe na HPLC, LC-MS, ELISA.

Irashobora kuba igeragezwa ryinshi AFB1, AFB2, AFG1, AFG2. Irakwiriye ibinyampeke, ibiryo, imiti yubushinwa, nibindi kandi bitezimbere ubuziranenge bwintangarugero.

Ohereza imeri kuri twe Amabwiriza yo Gukoresha

Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro ku bicuruzwa

Injangwe no.	KH01102Z
Ibyiza	Kuri Aflatoxin Ikizamini Cyuzuye
Aho byaturutse	Beijing, Ubushinwa
Izina ry'ikirango	Kwinbon
Ingano yubumwe	Ibizamini 25 kuri buri gasanduku
Icyitegererezo	Kugaburira, Ibinyampeke, Ibinyampeke n'ibirungo
Ububiko	2-30 ℃
Ubuzima bwa Shelf	Amezi 12
Gutanga	Icyumba cy'ubushyuhe

Ibikoresho & Reagents Birakenewe

Ibikoresho

Reagents

Ibikoresho

---- Homogenizer ---- Vortex ivanga

---- Icupa ry'icyitegererezo ---- Gupima silinderi: 10ml, 100ml

---- Impapuro zungurura impapuro / Centrifuge ---- Impuzandengo yisesengura (inductance: 0.01g)

---- Impamyabumenyi yarangije: 10ml ---- Injiza: 20ml

---- Amashanyarazi ya volumetric: 250ml ---- Rubber pipette

---- Micropipette: 100-1000ul ---- Glass funnel 50ml

---- Akayunguruzo ka Microfibre (Whatman, 934-AH, Φ11cm, 1.5um ruziga)

Reagents

---- Methanol (AR)

---- Acide acike (AR)

---- Sodium chloride (NACL, AR)

---- Amazi ya Deionised

Ibyiza byibicuruzwa

Kwinbon Inmmunoaffinity Inkingi ikoresha chromatografiya yamazi yo gutandukana, kwezwa cyangwa gusesengura byihariye bya Aflatoxin Igiteranyo. Mubisanzwe inkingi za Kwinbon zahujwe na HPLC.
Antibody ya monoclonal irwanya Aflatoxin Igiteranyo gifitanye isano nigitangazamakuru gikwirakwiza inkingi. Mycotoxine murugero ikuramo, kuyungurura no kuyungurura. Kora icyitegererezo cyo gukuramo igisubizo kinyura muri Aflatoxin Inkingi Yose. Ibisigazwa bya Aflatoxin (B1, B2, G1, G2) byahujwe na antibody ukwayo mu nkingi, igisubizo cyo gukaraba gikuraho umwanda udahujwe. Hanyuma, ukoresheje inzoga ya methyl kugirango uhoshe Aflatoxin B1, Aflatoxin B2, Aflatoxin G1, Aflatoxin G2.
Hamwe nibisobanuro bihanitse, inkingi ya Kwinbon AFT irashobora gufata molekile yintego muburyo bwiza cyane. Nanone inkingi za Kwinbon zitemba vuba, byoroshye gukora. Noneho birihuta kandi bikoreshwa cyane mubiryo no murima ya mycotoxine decetion.