Ubushinwa Inkingi Inkingi ya Aflatoxin Uruganda rwibinjira hamwe nabakora

Inkingi ya Immunoafficine kuri Aflatoxin Igishusho Cyuzuye

Inkingi ya Immunoafficine kuri Aflatoxin yose

Ibisobanuro bigufi:

Inkingi ya AFT ikoreshwa muguhuza na HPLC, LC-MS, Ikizamini cya Elisa.

Birashobora kuba byinshi kugerageza AFB1, AFB2, AFG1, AFG2. Birakwiriye ibinyampeke, ibiryo, imiti yubushinwa, nibindi kandi itezimbere ubuziranenge bwingero.

Ohereza imeri kuri twe Icyerekezo cyo gukoresha

Ibisobanuro birambuye

Ibicuruzwa

Injangwe oya.	Kh01102z
Umutungo	Kuri Aflatoxin Kwipimisha
Aho inkomoko	Beijing, Ubushinwa
Izina	Kwinbon
Ingano	Ibizamini 25 kuri buri gasanduku
Icyitegererezo	Kugaburira, ibinyampeke, ingano n'ibirungo
Ububiko	2-30 ℃
Imibereho-Ubuzima	Amezi 12
GUTANGA	Icyumba cyambere

Ibikoresho & reagent bisabwa

Ibikoresho

Reagent

Ibikoresho

---- homogenizer ---- Vortex mixer

---- Icumbi ---- Gupima Cylinder: 10ml, 100ml

---- Umunyarubuga Impapuro / Centrifuge ---- Impirimbanyi zisesengura (Inductance: 0.01g)

---- Umuyoboro wangiritse: 10ml ---- injiza: 20ml

---- Flask Flask: 250ml ---- Rubber piettte

---- Micropipette: 100-1000ul ---- Ikirahure cya 50ml

---- Akayunguruzo ka Microfiber (Whatman, 934-Ah, φ11cm, 1.5um Uruziga)

Reagent

---- methanol (ar)

---- aside acide (ar)

---- Chloride ya Sodium (Nacl, Ar)

---- amazi ya deionized

Ibyiza Byibicuruzwa

Kwinbon Inmmunoaffinity inkingi zikoresha chromatografiya yo gutandukana, kwezwa cyangwa gusesengura bimwe na kimwe cya Aflatoxin. Mubisanzwe inkingi ya Kwinbon ihujwe na HPLC.
Monoclonal antibods yo kurwanya Aflatoxin yose ihujwe nitangazamakuru rya coagula inkingi. Mycotoxines murugero irambuwe, yuruhuru kandi iravangwa. Kora igisubizo cyo gukuramo icyitegererezo kinyura muri aflatoxin yose. Aflatoxin (B1, B2, G1, G2) Ibisigisigi bihujwe na antibods ukwayo mu nkingi, igisubizo cyo gukaraba gikuraho umwanda kidahujwe. Hanyuma, ukoresheje inzoga za methyl kuri elute aflatoxin b1, Aflatoxin B2, Aflatoxin G1, Aflatoxin G2.
Hamwe nihariye, Kwinbon Aft Inkingi irashobora gufata molekile zigamije muburyo bwera cyane. Kandi inkingi ya Kwinbon igenda yihuta, Eassy gukora. Noneho birahunze kandi nkoresha cyane muburiganya no muri salle ya Mycotoxines.