Ubushinwa Immunoaffinity inkingi za Aflatoxin B1 uruganda rukora inganda nabakora

Immunoaffinity inkingi ya Aflatoxin B1 gutahura Ishusho Yerekanwe

Immunoaffinity inkingi za Aflatoxin B1 gutahura

Ibisobanuro bigufi:

Inkingi za Kwinbon Aflatoxin B1 zikoreshwa muguhuza hamwe na HPLC, LC-MS, ELISA. Irashobora kuba igipimo cyinshi AFB1 kubinyampeke, ibishyimbo nibicuruzwa byabo, amavuta yimboga namavuta, ibikomoka ku mbuto, isosi ya soya, vinegere, imiti yubushinwa, ibirungo nicyayi.

Ohereza imeri kuri twe Amabwiriza yo Gukoresha

Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro ku bicuruzwa

Injangwe no.	KH01104Z
Ibyiza	Kwipimisha Aflatoxin B1
Aho byaturutse	Beijing, Ubushinwa
Izina ry'ikirango	Kwinbon
Ingano yubumwe	Ibizamini 25 kuri buri gasanduku
Icyitegererezo	Ibinyampeke, ibishyimbo n'ibicuruzwa byabo, amavuta y'ibimera n'amavuta, ibikomoka ku mbuto, isosi ya soya, vinegere, imiti y'Ubushinwa, ibirungo n'icyayi
Ububiko	2-30 ℃
Ubuzima bwa Shelf	Amezi 12
Gutanga	Icyumba cy'ubushyuhe

Ibikoresho & Reagents Birakenewe

Ibikoresho

Reagents

Ibikoresho

---- Homogenizer ---- Vortex ivanga

---- Icupa ry'icyitegererezo ---- Gupima silinderi: 10ml, 100ml

---- Impapuro zungurura impapuro / Centrifuge ---- Impuzandengo yisesengura (inductance: 0.01g)

---- Impamyabumenyi yarangije: 10ml ---- Injiza: 20ml

---- Amashanyarazi ya volumetric: 250ml ---- Rubber pipette

---- Micropipette: 100-1000ul ---- Glass funnel 50ml

---- Akayunguruzo ka Microfibre (Whatman, 934-AH, Φ11cm, 1.5um ruziga)

Reagents

---- Methanol (AR)

---- Acide acike (AR)

---- Sodium chloride (NACL, AR)

---- Amazi ya Deionised

Ibyiza byibicuruzwa

Aflatoxin B1 ni umwanda ukabije mu biribwa bitandukanye birimo ibishyimbo, ifunguro ry'imbuto, ibigori, n'ibindi binyampeke; kimwe no kugaburira amatungo. Aflatoxin B1 ifatwa nka aflatoxine ifite ubumara bwinshi kandi ifite uruhare runini muri kanseri y'umwijima (HCC) mu bantu.

Wikipedia irasaba uburyo bukurikira bwo gutahura;

Inzira ya chromatografiya
Enzyme-ihuza immunosorbent assay
Immunoaffinity inkingi fluorescence
Immunoaffinity inkingi ikora neza ya chromatografiya

Kwinbon Inmmunoaffinity Inkingi nuburyo bwa gatatu, ikoresha chromatografiya yamazi yo gutandukana, kweza cyangwa gusesengura byihariye bya Aflatoxin B1. Mubisanzwe inkingi za Kwinbon zahujwe na HPLC.

HPLC isesengura ryinshi ryuburozi bwa fungal nubuhanga bukuze bwo kumenya. Byombi imbere na revers ya chromatografi irakoreshwa. Guhindura icyiciro HPLC nubukungu, byoroshye gukora, kandi bifite uburozi buke. Uburozi bwinshi burashonga mubice byimikorere ya polar hanyuma bigatandukanywa ninkingi za chromatografiya zidafite inkingi, byujuje ibisabwa kugirango hamenyekane vuba uburozi bwinshi bwibihumyo mubyitegererezo byamata. UPLC ihuriweho na disiketi igenda ikoreshwa buhoro buhoro, hamwe ningutu yumuvuduko mwinshi hamwe nubunini buto hamwe nubunini bwa chromatografiya, bishobora kugabanya icyitegererezo cyo gukora, kunoza imikorere ya chromatografique, kandi bikagera no kubyumva neza.

Hamwe nibisobanuro bihanitse, Kwinbon Aflatoxin B1 inkingi irashobora gufata molekile yintego muburyo bwiza cyane. Nanone inkingi za Kwinbon zitemba vuba, byoroshye gukora. Noneho birihuta kandi bikoreshwa cyane mubiryo no murima ya mycotoxine decetion.