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ibicuruzwa

Elisa Ikizamini cya Ochratoxin Igishusho cyihariye
  • Elisa Ikizamini cya Ochratoxin A.
  • Elisa Ikizamini cya Ochratoxin A.

Elisa Ikizamini cya Ochratoxin A.

Ibisobanuro bigufi:

Iki gikoresho kirashobora gukoreshwa mubisesengura ryinshi kandi ryujuje ubuziranenge bwa ochratoxine A mubiryo.Nibicuruzwa bishya byo kumenya ibisigazwa byibiyobyabwenge bishingiye ku ikoranabuhanga rya ELISA, bigura 30min gusa muri buri gikorwa kandi birashobora kugabanya cyane amakosa yibikorwa ndetse nimbaraga zakazi.Iki gikoresho gishingiye ku buryo butaziguye tekinoroji ya ELISA.Amariba ya microtiter yashizwemo na antigen ihuza.Ochratoxin A murugero irushanwa na antigen yometse ku isahani ya microtiter kugirango ntamuntu wongeyeho.Nyuma yo kongeramo enzyme conjugate, TMB substrate ikoreshwa mukwerekana ibara.Gukuramo icyitegererezo bifitanye isano ribi na o chratoxine Igisigisigi cyacyo, nyuma yo kugereranya na Standard Curve, igwizwa nimpamvu zidindiza, Ochratoxin Umubare murugero urashobora kubarwa.


Amabwiriza yo Gukoresha

Ibicuruzwa birambuye

Ibicuruzwa

Ochratoxine ni itsinda rya mycotoxine ikorwa nubwoko bumwe na bumwe bwa Aspergillus (cyane cyane A).Ochratoxin A izwiho kugaragara mubicuruzwa nk'ibinyampeke, ikawa, imbuto zumye na vino itukura.Ifatwa nka kanseri yumuntu kandi ifite inyungu zidasanzwe kuko ishobora kwegeranywa mu nyama zinyamaswa.Rero inyama ninyama zirashobora kwanduzwa nubu burozi.Guhura na ochratoxine binyuze mumirire birashobora kugira uburozi bukabije bwimpyiko zinyamabere, kandi birashobora kuba kanseri.

Deatail

1. Ikizamini cya Elisa cya Ochratoxin A.

2. Injangwe.KA07301H-96 Iriba

3. Ibigize ibikoresho
Pl Isahani ya Microtiter ifite amariba 96 yometse kuri antigen
Solutions Ibisubizo bisanzwe (amacupa 6: 1ml / icupa)
0ppb, 0.4ppb, 0.8ppb, 1.6ppb, 3.2ppb, 6.4ppb
● Enzyme conjugate 7ml ……………………………………………………………… .. ……… ..… ..umutwe
Solution Igisubizo cya Antibody 10ml ………………………………………………………………… ...….… Cap cap
Gukuramo igisubizo A 7ml ……………………………………………………………………………… ingofero yera
Sub Substrate Solution B 7ml ………………………………………………………… .. ………………… umutuku
Hagarika igisubizo 7ml ……………………………………………………………. …………………… ingofero yumuhondo
● 20 × yibanze Gukaraba igisubizo 40ml ……… .. ………………………………….… ...… capa ibonerana

4. Ibyiyumvo, ukuri kandi neza
Kwiyumvisha Ikizamini: 0.4ppb
Imipaka ntarengwa
Kugaburira …………………………………………………. ……… .. …………….… 5ppb
Ukuri
Kugaburira …………………………………………………………. ……….….… 90 ± 20%
Icyitonderwa:Coefficient yo gutandukanya ibikoresho bya ELISA iri munsi ya 10%.

5. Igipimo cy'umusaraba
Ochratoxin A ……………………………………………… .. ……………… ..100%

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