ibicuruzwa

Ibyerekeye

Iki gikoresho kirashobora gukoreshwa mubisesengura ryinshi kandi ryujuje ubuziranenge bwa ochratoxine A mubiryo.Nibicuruzwa bishya byo kumenya ibisigazwa byibiyobyabwenge bishingiye ku ikoranabuhanga rya ELISA, bigura 30min gusa muri buri gikorwa kandi birashobora kugabanya cyane amakosa yibikorwa ndetse nimbaraga zakazi.Iki gikoresho gishingiye ku buryo butaziguye tekinoroji ya ELISA.Amariba ya microtiter yashizwemo na antigen ihuza.Ochratoxin A murugero irushanwa na antigen yometse ku isahani ya microtiter kugirango ntamuntu wongeyeho.Nyuma yo kongeramo enzyme conjugate, TMB substrate ikoreshwa mukwerekana ibara.Gukuramo icyitegererezo bifitanye isano ribi na o chratoxine Igisigisigi cyacyo, nyuma yo kugereranya na Standard Curve, igwizwa nimpamvu zidindiza,Ochratoxin Ingano murugero irashobora kubarwa.

Ibigize ibikoresho

Isahani ya Microtiter ifite amariba 96 yometse kuri antigen

•Sibisubizo bya tandard (amacupa 6: 1ml / icupa)

0ppb, 0.4ppb, 0.8ppb, 1.6ppb, 3.2ppb, 6.4ppb

Enzymeconjugate7ml ……………………………………………………………….....…..umutuku

• Umuti wa Antibody10ml ………………………………………………………………………...….Cap icyatsi kibisi

•Substrate solution A 7ml ……………………………………………………………………

•SubstrateIgisubizo B 7ml …………………………………………………………..Umutwe utukura

• Hagarika igisubizo 7ml ………………………………………………………………….Umutwe w'umuhondo

• 20 × yibanze Gukaraba igisubizo 40ml ………..………………………………….…...Cap cap

Ibyiyumvo, ukuri kandi neza

Kwiyumvisha Ikizamini: 0.4ppb

Imipaka ntarengwa

Kugaburira ………………………………………………….……… .. …………….… 5ppb

Ukuri

Kugaburira ………………………………………………………….……….….… 90 ± 20%

Icyitonderwa

Coefficient yo gutandukanya ibikoresho bya ELISA iri munsi ya 10%.

Igipimo cy'umusaraba

Ochratoxin A ………………………………………………..……………… ..100%