Elisa Ikizamini cya AOZ
Nitrofurans ni antibiyotike ya sintetike yagutse, ikoreshwa cyane mu musaruro w’inyamanswa kubera antibacterial na farumasi nziza.
Zari zarakoreshejwe kandi mu guteza imbere ingurube, inkoko n’umusaruro w’amazi.Mu bushakashatsi bwigihe kirekire hamwe ninyamaswa zo muri laboratoire bwerekanye ko imiti yababyeyi na metabolite yabo yerekanaga kanseri na mutagenic.Imiti ya nitrofuran furaltadone, nitrofurantoin na nitrofurazone yabujijwe gukoresha mu gutunganya amatungo y’ibiribwa muri EU mu 1993, kandi byari bibujijwe gukoresha furazolidone mu 1995.
Ibisobanuro
1.Elisa Ikizamini cya AOZ
2.Cat.A008-96 Iriba
3.Ibikoresho
Pl Isahani ya microtiter yometse kuri antigen, amariba 96
Solutions Ibisubizo bisanzwe (amacupa 6, 1ml / icupa)
0ppb, 0.025ppb, 0.075ppb, 0.225ppb, 0.675ppb, 2.025ppb
Igenzura risanzwe rigenzura: (1ml / icupa) ........................................ ........… .100ppb
● Enzyme conjugate yibanze 1.5ml ............................................ ..….… .Rap
Solution Antibody solution yibanze 0.8ml …………………………… ....… cap cap
Gukuramo A 7ml ……… ........................................... ..............… ....… ..… ..umutwe wera
Substrate B7ml ……… ..............… ............................. ....................… ..… ..umutwe
Hagarika igisubizo 7ml ………………………………………………. ……… cap cap yumuhondo
● 20 × yibanze yo gukaraba 40ml ………………………. …… capa ibonerana
2
● 2-Nitrobenzaldehyde 15.1mg …………………………………………… ingofero yumukara
4.Ibyiyumvo, ukuri kandi neza
Ibyiyumvo: 0.025ppb
Imipaka ntarengwa …………… .. ………………………… 0.1ppb
Ukuri:
Inyama zinyamaswa (imitsi numwijima) ………. ………… 75 ± 15%
Ubuki ……………………………… .. ……………… ..90 ± 20%
Amagi ………………………………… .. …………… ..… 90 ± 20%
Amata ………………………………… .. …………… ..… 90 ± 10%
Icyitonderwa: CV yigikoresho cya ELISA kiri munsi ya 10%.
5.Ikigereranyo rusange
Furazolidone metabolite (AOZ) …………………………. …………… ..100%
Furaltadone metabolite (AMOZ) …………………………. …………… <0.1%
Nitrofurantoin metabolite (AHD) ……………………………………… <0.1%
Nitrofurazone metabolite (SEM) ……………………. …………… ...… <0.1%
Furazolidone …………………………………………. ………….… ..… 16.3%
Furaltadone ………………………………………. ………………….… <1%
Nitrofurantoin …………………………………………………. …….… <1%
Nitrofurazone ……………………………………. …………………… ..… <1%