ELisa Ikizamini cya AOZ
Ibyerekeye
Iki gikoresho gishobora gukoreshwa mu isesengura ryinshi kandi ryujuje ubuziranenge bwa AOZ mu nyama z’inyamaswa (inkoko, inka, ingurube, nibindi), amata, ubuki n'amagi.
Isesengura ry’ibisigazwa by’ibiyobyabwenge bya nitrofuran bigomba gushingira ku gutahura metabolite ya tissue ihuza imiti y’ababyeyi ba nitrofuran, irimo metabolite ya Furazolidone (AOZ), metabolite ya Furaltadone (AMOZ), metabolite ya Nitrofurantoin (AHD) na Nitrofurazone metabolite (SEM).
Ugereranije nuburyo bwa chromatografique, ibikoresho byacu byerekana ibyiza byinshi bijyanye na sensitivite, imipaka yo gutahura, ibikoresho bya tekiniki nibisabwa igihe.
Ibigize ibikoresho
Isahani ya Microtiter yometse kuri antigen, amariba 96
• Ibisubizo bisanzwe (amacupa 6, 1ml / icupa)
0ppb, 0.025ppb, 0.075ppb, 0.225ppb, 0.675ppb, 2.025ppb
• Kugenzura kugenzura bisanzwe: (1ml / icupa) .................................................100ppb
• Enzyme conjugate yibanze 1.5ml ..............................................….… .Rap
• Umuti wa Antibody wibanze 0.8ml …………………………….... Cap icyatsi kibisi
• Gukuramo A 7ml ……… .........................................................… ....… ..… ..umutwe wera
• Substrate B7ml ……… ..............… .................................................… ..… ..umutwe
• Hagarika igisubizo 7ml ……………………………………………….Cap Umuhondo
• 20 × yibanze yo gukaraba 40ml ………………………. …… capa ibonerana
• 2 solution igisubizo gikurura ibisubizo 60ml …………………...Urupapuro
• 2-Nitrobenzaldehyde 15.1mg …………………………………………… ingofero yumukara
Ibyiyumvo, ukuri kandi neza
Ibyiyumvo: 0.025ppb
Imipaka ntarengwa…………… .. ………………………… 0.1ppb
Ukuri:
Inyama zinyamaswa (imitsi numwijima) ………. ………… 75 ± 15%
Ubuki ……………………………… .. ……………… ..90 ± 20%
Amagi ………………………………… .. …………… ..… 90 ± 20%
Amata ………………………………… .. …………… ..… 90 ± 10%
Icyitonderwa:CV ya ELISA kit kiri munsi ya 10%.
Igipimo cy'umusaraba
Furazolidone metabolite (AOZ) …………………………. …………… ..100%
Furaltadone metabolite (AMOZ) …………………………. …………… <0.1%
Nitrofurantoin metabolite (AHD) ……………………………………… <0.1%
Nitrofurazone metabolite (SEM) ……………………. …………… ...… <0.1%
Furazolidone …………………………………………. ………….… ..… 16.3%
Furaltadone ………………………………………. ………………….… <1%
Nitrofurantoin …………………………………………………. …….… <1%
Nitrofurazone ……………………………………. …………………… ..… <1%