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Elisa Ikizamini cya Aflatoxin B1 Ishusho Yerekanwe
  • Ikizamini cya Elisa cya Aflatoxin B1
  • Ikizamini cya Elisa cya Aflatoxin B1
  • Ikizamini cya Elisa cya Aflatoxin B1

Ikizamini cya Elisa cya Aflatoxin B1

Ibisobanuro bigufi:

Umubare munini wa aflatoxine utera uburozi bukabije (aflatoxicose) bushobora guhitana ubuzima, akenshi binyuze mu kwangiza umwijima.

Aflatoxin B1 ni aflatoxine ikorwa na Aspergillus flavus na A. parasitike.Ni kanseri ikomeye cyane.Izi mbaraga za kanseri ziratandukanye bitewe nubwoko bumwe na bumwe, nk'imbeba n'inguge, bisa nkaho byoroshye kurusha ibindi.Aflatoxin B1 ni umwanda ukabije mu biribwa bitandukanye birimo ibishyimbo, ifunguro ry'imbuto, ibigori, n'ibindi binyampeke;kimwe no kugaburira amatungo.Aflatoxin B1 ifatwa nka aflatoxine ifite ubumara bukabije kandi ifite uruhare runini muri kanseri ya hepatocellular kanseri (HCC) mu bantu.Uburyo bwinshi bwo gutoranya no gusesengura harimo chromatografi yoroheje (TLC), chromatografiya ikora cyane (HPLC), mass spectrometrie, hamwe na enzyme ihujwe na immunosorbent assay (ELISA), nibindi, byakoreshejwe mugupima kwanduza aflatoxine B1 mubiribwa .Ishami ry’ibiribwa n’ubuhinzi (FAO) rivuga ko ku isi hose urugero rwa aflatoxine B1 rwihanganirwa ku isi hose ruri hagati ya 1–20 μg / kg mu biribwa, na 5-50 μg / kg mu biryo by’inka by’ibiryo mu 2003.


Amabwiriza yo Gukoresha

Ibicuruzwa birambuye

Ibicuruzwa

Ibyerekeye

Iki gikoresho kirashobora gukoreshwa mubisesengura ryujuje ubuziranenge na aflatoxine B1 mu mavuta aribwa, ibishyimbo, ibinyampeke, isosi ya soya, vinegere hamwe n ibiryo (ibiryo bibisi, ibikoresho bivanze hamwe nibikoresho byibanze). isesengura ry'ibikoresho.

Iki gicuruzwa gishingiye ku buryo butaziguye ELISA irushanwa, yihuta, yuzuye kandi yoroheje ugereranije nisesengura ryibikoresho bisanzwe.Irakeneye 45min gusa mugikorwa kimwe, gishobora kugabanya cyane amakosa yibikorwa hamwe nimbaraga zakazi.

Ibigize ibikoresho

• Microtiter yashizwemo antigen, amariba 96

• Igisubizo gisanzwe × 6 Icupa (1ml / icupa)

0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb

• Enzyme conjugate 7ml …………………………………………………………… .. cap cap cap itukura

• Umuti wa Antibody7ml ................................................................. ................. cap icyatsi kibisi

• Gukuramo A 7ml ………………………………………………………….………… ...… ingofero yera

• Substrate B 7ml ………………. ……………………………………………….Ingofero itukura

• Hagarika igisubizo 7ml ………. ……. ……………………………………………….. Cap Umuhondo

• 20 × yibanze yo gukaraba 40ml ……………………………………

• 2 × gukemura cyane ibisubizo 50ml cap cap cap yubururu

Ibyiyumvo, ukuri kandi neza

Ibyiyumvo:0.05ppb

Imipaka ntarengwa

Icyitegererezo cyamavuta yo kurya ............................................... .......................................................................... 0.1ppb

Ibishyimbo ................................................. .................................................. ....................... 0.2ppb

Ibinyampeke ................................................. .................................................. ...................... 0.05ppb

Ukuri

Icyitegererezo cyamavuta yo kurya ............................................... ...................................................................... 80 ± 15%

Ibishyimbo ................................................. .................................................. ..................... 80 ± 15%

Ibinyampeke ................................................. .................................................. ..................... 80 ± 15%

Icyitonderwa

Coefficient yo gutandukanya ibikoresho bya ELISA iri munsi ya 10%.

Igipimo cy'umusaraba

Aflatoxin B.1··················· 100%

Aflatoxin B2 ······················.%

Aflatoxin G1 ····················· 62%

Aflatoxin G2 ······················· 22.3%

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