This ELISA kit is designed to detect quinolones based on the principle of indirect-competitive enzyme immunoassay. The microtiter wells are coated with capture BSA-linked antigen. Quinolones in the sample competes with antigen coated on the microtitre plate for the antibody. After the addition of enzyme conjugate, chromogenic substrate is used and the signal is measured by spectrophotometer. The absorption is inversely proportional to the quinolones concentration in the sample.
