The AFT-DON-ZEN-OTA 7 in 1 immunoaffinity column can absorb the total Aflatoxin(AFB1, AFB2, AFG1, AFG2), deoxynivalenol (DON), zearalenone(ZEN) and Ochratoxin A(OTA) in the sample extraction when the sample solution get through this immunoaffinity columns. It can enrich and purify four kinds of mycotoxins. Compared with the single immunoaffinity column, it has the advantage of improving work efficiency and saving cost. The purified extraction can also be detected simultaneously by the same analytical method.