mankhwala

Ma nitrofuran amapangidwa m'thupi mwachangu kwambiri, ndipo ma metabolites awo ophatikizidwa ndi minyewa amakhalapo kwa nthawi yayitali, kotero kusanthula kotsalira kwa mankhwalawa kudzadalira kuzindikira kwa metabolites yawo, kuphatikiza furazolidone metabolite (AOZ), furazolidone metabolite (AMOZ). ), nitrofurantoin metabolite (AHD) ndi nitrofurazone metabolite (SEM).

Kwinbon Competitive Enzyme Immunoassay kits, yomwe imadziwikanso kuti Elisa kits, ndiukadaulo wa bioassay potengera mfundo ya Enzyme-Linked Immunosorbent Assay (ELISA). Ubwino wake umawonekera makamaka muzinthu izi:

 (1) Kuthamanga: Kawirikawiri ma laboratory amatenga LC-MS ndi LC-MS/MS kuti azindikire metabolite ya nitrofurazone. Komabe mayeso a Kwinbon ELISA, momwe ma antibody enieni a SEM amatuluka ndi olondola, omvera, komanso osavuta kugwiritsa ntchito. Nthawi yoyeserera ya zida izi ndi 1.5h yokha, yomwe imakhala yothandiza kwambiri kuti mupeze zotsatira. Izi ndizofunikira kuti muzindikire mwachangu komanso kuchepetsa kuchuluka kwa ntchito.

(2) Kulondola: Chifukwa cha kutsimikizika kwakukulu komanso kukhudzidwa kwa zida za Kwinbon SEM Elisa, zotsatira zake ndi zolondola kwambiri ndi zolakwika zochepa. Izi zimathandiza kuti zigwiritsidwe ntchito kwambiri m'ma laboratories azachipatala ndi m'mabungwe ofufuza kuti athandize minda yausodzi ndi ogulitsa zinthu zam'madzi pozindikira ndikuyang'anira zotsalira zamankhwala azinyama za SEM muzinthu zam'madzi.

(3) Kukhazikika kwapamwamba: Kwinbon SEM Elisa kit ili ndi mawonekedwe apamwamba ndipo imatha kuyesedwa motsutsana ndi ma antibodies enieni. Mtanda wa SEM ndi metabolite yake ndi 100%. Corss reaction imawonetsa kuchepera 0.1% ya AOZ, AMOZ, AHD, CAP ndi ma metabolites awo, Imathandiza kupewa kuzindikiridwa molakwika ndi kusiyidwa.