China Immunoaffinity mizati kwa Zearalenone kudziwika fakitale ndi opanga

Magawo a Immunoaffinity a kuzindikira kwa Zearalenone Chithunzi Chowonetsedwa

Mipingo ya Immunoaffinity ya kuzindikira kwa Zearalenone

Kufotokozera Kwachidule:

Zipilala za Kwinbon Zearalenone zimagwiritsidwa ntchito pophatikiza ndi HPLC, LC-MS, ELISA test kit. Kutha kukhala kuyesa kochulukira kwa AFB1 pambewu ndi zinthu zambewu, msuzi wa soya, viniga, zinthu za msuzi, mowa, soya, mafuta ophatikizika ndi masamba, kudyetsa zopangira ndi zomalizidwa, ndi zina zambiri.

Titumizireni imelo Malangizo Ogwiritsa Ntchito

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Mafotokozedwe azinthu

Mphaka no.	KH00304Z
Katundu	ZaZearalenonekuyesa
Malo Ochokera	Beijing, China
Dzina la Brand	Kwinbon
Kukula kwa Unit	25 mayesero pa bokosi
Chitsanzo cha Ntchito	Mbewu ndi zinthu zambewu, msuzi wa soya, viniga, zopangira msuzi, mowa, soya, mafuta ophatikizika ndi masamba, kudyetsa zopangira ndi zinthu zomalizidwa, etc.
Kusungirako	2-30 ℃
Alumali moyo	Miyezi 12
Kutumiza	Kutentha kwachipinda

Zida & Reagents Zofunika

Zida

Ma reagents

Zida

----Homogenizer ---- Vortex chosakanizira

----Botolo lachitsanzo ----Silinda yoyezera: 10ml, 100ml

----Pepala losefera labwino/Centrifuge -----Kuwerengera bwino (kulowetsa: 0.01g)

----Pipette yomaliza maphunziro: 10ml ----Injector: 20ml

----Botolo la volumetric: 250ml ----Babu la rabara la pipette

----Micropipette: 100-1000ul ----Faneli yagalasi 50ml

----Zosefera za Microfiber (Whatman, 934-AH, Φ11cm, 1.5um circle)

Ma reagents

Methanol (AR)

----Acetic acid (AR)

----Sodium chloride (NACL,AR)

----Madzi osungunuka

Ubwino wa mankhwala

Zearalenone (ZEN), yomwe imadziwikanso kuti RAL ndi F-2 mycotoxin, ndi metabolite yamphamvu ya estrogenic yopangidwa. Sizitentha kwambiri ndipo imapezeka padziko lonse m’mbewu zosiyanasiyana monga chimanga, balere, oats, tirigu, mpunga, ndi manyuchi.

Zearalenone ndiye poizoni wamkulu yemwe amamangiriza ku zolandilira za estrogen, zomwe zimayambitsa kusabereka, kuchotsa mimba kapena zovuta zina zoswana, makamaka mu nkhumba.

Wikipedia imalimbikitsa njira zodziwira zotsatirazi;

Thin layer chromatography,
Kuyesa kwa enzyme yolumikizana ndi immunosorbent
Immunoaffinity column fluorescence
Immunoaffinity column high performance liquid chromatography

Kwinbon Inmmunoaffinity Columns ndi njira yachitatu, imagwiritsa ntchito chromatography yamadzimadzi pakulekanitsa, kuyeretsa kapena kusanthula kwapadera kwa Zearalenone. Nthawi zambiri mizati ya Kwinbon imaphatikizidwa ndi HPLC.

HPLC kusanthula kuchuluka kwa poizoni mafangasi ndi njira yodziwira okhwima. Ma chromatography onse opita patsogolo ndi kumbuyo akugwira ntchito. Reverse phase HPLC ndiyopanda ndalama, yosavuta kugwiritsa ntchito, ndipo ili ndi kawopsedwe kakang'ono ka zosungunulira. Poizoni ambiri amasungunuka m'magawo amtundu wa polar kenako amasiyanitsidwa ndi mizati ya non-polar chromatography, zomwe zimakwaniritsa zofunikira kuti zizindikire mwachangu poizoni wa mafangasi muzakudya za mkaka. Ofufuza a UPLC amagwiritsidwa ntchito pang'onopang'ono, module ndi ma module apamwamba komanso kukula kwa zigawo zazing'ono.

Ndi kutsimikizika kwakukulu, mizati ya Kwinbon Zearalenone imatha kugwira mamolekyu omwe ali mumkhalidwe woyera kwambiri. Komanso mizati ya Kwinbon imayenda mwachangu, yosavuta kugwira ntchito. Tsopano ikugwiritsidwa ntchito mwachangu komanso mofala m'munda wa chakudya ndi tirigu pachinyengo cha mycotoxins.