China Immunoaffinity mizati kwa Ochratoxin A kudziwika fakitale ndi opanga

Mizati ya Immunoaffinity ya Kuzindikira kwa Ochratoxin A Chithunzi Chowonetsedwa

Mipingo ya Immunoaffinity ya kuzindikira kwa Ochratoxin A

Kufotokozera Kwachidule:

Ma column a Kwinbon Ochratoxin A amagwiritsidwa ntchito pophatikiza ndi HPLC, LC-MS, ndi ELISA test kit. Kungakhale kuyesa kuchuluka kwa Ochratoxin A pogwiritsa ntchito tirigu ndi zinthu za tirigu, soya sauce, viniga, zinthu za msuzi, mowa, Cocoa ndi khofi wokazinga, ndi zina zotero.

Titumizireni imelo Malangizo Ogwiritsa Ntchito

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Mafotokozedwe azinthu

Mphaka no.	KH00404Z
Katundu	ZaOchratoxin A kuyesa
Malo Ochokera	Beijing, China
Dzina la Brand	Kwinbon
Kukula kwa Unit	Mayeso 25 pa bokosi lililonse
Chitsanzo cha Ntchito	Gzinthu zamvula ndi tirigu, soya msuzi, viniga, zinthu za msuzi, mowa, Cocoa ndi khofi wokazinga, ndi zina.
Kusungirako	2-30 ℃
Alumali moyo	Miyezi 12
Kutumiza	Kutentha kwachipinda

Zida & Reagents Zofunika

Zida

Ma reagents

Zida

----Homogenizer -----Vortex chosakanizira

----Botolo lachitsanzo ----Silinda yoyezera: 10ml, 100ml

----Pepala losefera labwino/Centrifuge -----Kuwerengera bwino (kulowetsa: 0.01g)

----Pipette yomaliza maphunziro: 10ml ----Injector: 20ml

----Botolo la volumetric: 250ml ----Babu la rabara la pipette

----Micropipette: 100-1000ul ----Faneli yagalasi 50ml

----Zosefera za Microfiber (Whatman, 934-AH, Φ11cm, 1.5um circle)

Ma reagents

Methanol (AR)

----Acetic acid (AR)

----Sodium chloride (NACL,AR)

----Madzi oyeretsedwa

Ubwino wa malonda

Monga amadziwika kuti mycotoxin, Ochratoxin A (OTA) yopangidwa ndi mitundu ingapo ya mafangasi kuphatikiza Aspergillus ochraceus, A. carbonarius, A. niger ndi Penicillium verrucosum. OTA imayambitsa nephrotoxicity ndi zotupa za aimpso mumitundu yosiyanasiyana ya nyama; komabe, zotsatira za thanzi la anthu sizidziwika bwino.

Kwinbon Inmmunoaffinity Columns ndi njira yachitatu, imagwiritsa ntchito chromatography yamadzimadzi pakulekanitsa, kuyeretsa kapena kusanthula kwapadera kwa Ochratoxin A. Nthawi zambiri mizati ya Kwinbon imaphatikizidwa ndi HPLC.

HPLC kusanthula kuchuluka kwa poizoni mafangasi ndi njira yodziwira okhwima. Ma chromatography onse opita patsogolo ndi kumbuyo akugwira ntchito. Reverse phase HPLC ndiyopanda ndalama, yosavuta kugwiritsa ntchito, ndipo ili ndi kawopsedwe kakang'ono ka zosungunulira. Poizoni ambiri amasungunuka m'magawo amtundu wa polar kenako amasiyanitsidwa ndi mizati ya non-polar chromatography, zomwe zimakwaniritsa zofunikira kuti zizindikire mwachangu poizoni wa mafangasi muzakudya za mkaka. Ofufuza a UPLC amagwiritsidwa ntchito pang'onopang'ono, module ndi ma module apamwamba komanso kukula kwa zigawo zazing'ono.

Ndi kutsimikizika kwapamwamba, mizati ya Kwinbon Ochratoxin A imatha kugwira mamolekyu omwe akuwatsata ali oyera kwambiri. Komanso mizati ya Kwinbon imayenda mwachangu, yosavuta kugwira ntchito. Tsopano ikugwiritsidwa ntchito mwachangu komanso mofala m'munda wa chakudya ndi tirigu pachinyengo cha mycotoxins.