mankhwala

Za

Izi zida ntchito mofulumira Mkhalidwe kusanthula tetracyclines uchi chitsanzo.

Chitsanzo chokonzekera njira;

  (1) Ngati uchi chitsanzo crystallized, kutenthetsa mu osamba madzi osapitirira 60 ℃, mpaka uchi chitsanzo thaw, kusakaniza kwathunthu, kuzirala monga firiji, ndiye kulemera kwa assay.

(2) Yesani 1.0 ± 0.05g homogenate mu chubu cha 10ml polystyrene centrifuge, onjezerani 3ml chitsanzo chotsitsa njira, vortex kwa 2min kapena gwedezani ndi dzanja mpaka chitsanzocho chisakanizidwe kwathunthu.

Zoyeserera.

(1.) Tengani mabotolo ofunikira mu phukusi la zida, chotsani makadi ofunikira, ndipo lembani zizindikiro zoyenera.Chonde gwiritsani ntchito makhadi oyeserera pasanathe 1h mukatsegula phukusi.

(2.) Tengani 100ml okonzeka chitsanzo mu dzenje chitsanzo ndi pipette, ndiye yambani nthawi pambuyo madzi otaya..

(3.) Yalirani kwa 10min pa kutentha kwa firiji.

LOD

 Zotsatira

Pali mizere iwiri muzotsatira zamakhadi,Mzere wowongolerandiTetracylcines Line, omwe amalembedwa mwachidule ngati "B” ndi “T”.Zotsatira za mayeso zidzadalira mtundu wa mizere iyi.Chithunzi chotsatirachi chikufotokoza chizindikiritso cha zotsatira.

Zoipa: Mzere Wowongolera ndi Mzere Woyesera onse ndi ofiira ndipo T Line ndi mdima kuposa mzere wolamulira;

Tetracyclines zabwino: Control Line ndi yofiira, T Line ilibe mtundu kapena T Line ndi yopepuka kuposa C line, kapena T Line ndi yofanana ndi C Line.