Elisa Test Kit ya Ochratoxin A
Za
Zidazi zitha kugwiritsidwa ntchito posanthula kuchuluka komanso kukwanira kwa ochratoxin A muzakudya.Ndi chinthu chatsopano chozindikira zotsalira za mankhwala pogwiritsa ntchito ukadaulo wa ELISA, womwe umangotengera 30min pa opareshoni iliyonse ndipo ukhoza kuchepetsa kwambiri zolakwika zogwirira ntchito komanso kuchuluka kwa ntchito.Chida ichi chimachokera paukadaulo wampikisano wa ELISA.Zitsime za microtiter zimakutidwa ndi antigen yolumikizana.Ochratoxin A mu zitsanzo amapikisana ndi antigen yokutidwa pa mbale ya microtiter ya antibody yomwe yawonjezeredwa.Pambuyo pakuwonjezera kwa enzyme conjugate, gawo lapansi la TMB limagwiritsidwa ntchito kuwonetsa mtundu.Kusapezeka kwachitsanzo kumakhudzana ndi o chratoxin A zotsalira m'menemo, mutafanizira ndi Standard Curve, kuchulukitsidwa ndi zinthu zowonongeka,Ochratoxin Kuchuluka mu zitsanzo kungawerengedwe.
Zida Zamagetsi
• mbale ya Microtiter yokhala ndi zitsime 96 zokutidwa ndi antigen
•Snjira wamba (6 mabotolo: 1ml/botolo)
0 ppb, 0.4ppb, 0.8ppb, 1.6ppb, 3.2ppb, 6.4ppb
• Enzymeconjugate7ml………………………………………………………………………..…………..…..kapu wofiira
• Njira yothetsera ma antibodies10ml…………………………………………………………………………...….…chipewa chobiriwira
•Gawo smlingo A 7ml………………………………………………………………………………… kapu yoyera
•Gawo lapansiSolution B 7ml ………………………………………………………………..…………………kapu yofiyira
• Stop solution 7ml ………………………………………………………………….……………………yellow cap
• 20×ayikira Sambani njira 40ml ………..………………………………………….…...…kapu yowonekera
Sensitivity, kulondola ndi kulondola
Kuzindikira Kuyesa: 0.4ppb
Malire ozindikira
Feed …………………………………………………………….………………………………… 5ppb
Kulondola
Feed …………………………………………………………………….………….….…90±20%
Kulondola
Kusiyanasiyana kwa zida za ELISA ndizochepera 10%.
Cross Rate
Ochratoxin A………………………………………………………..…………………..100%