Elisa Test Kit ya AOZ
Nitrofuran ndi mankhwala opha tizilombo tosiyanasiyana, omwe amagwiritsidwa ntchito kaŵirikaŵiri popanga zinyama chifukwa cha mankhwala ake abwino kwambiri a antibacterial ndi pharmacokinetic.
Amagwiritsidwanso ntchito ngati kulimbikitsa kukula kwa nkhumba, nkhuku ndi kupanga zam'madzi.M'kupita kwa nthawi, maphunziro a labu nyama anasonyeza kuti makolo mankhwala ndi metabolites awo anasonyeza carcinogenic ndi mutagenic makhalidwe.Mankhwala a nitrofuran a furaltadone, nitrofurantoin ndi nitrofurazone analetsedwa kugwiritsidwa ntchito popanga nyama ku EU mu 1993, ndipo kugwiritsa ntchito furazolidone kunali koletsedwa mu 1995.
Tsatanetsatane
1.Elisa Test Kit ya AOZ
2.Mphaka.A008-96 Zitsime
3.Kit Components
● mbale ya Microtiter yokutidwa ndi antigen, zitsime 96
● Standard solutions(mabotolo 6,1ml/botolo)
0ppb, 0.025ppb, 0.075ppb, 0.225ppb, 0.675ppb, 2.025ppb
● Spiking standard control : (1ml/botolo)............................................ .............100ppb
● Enzyme conjugate concentrate 1.5ml............................................ ..….….kapu yofiyira
● Antibody solution yakhazikika 0.8ml………………………………..kapu yobiriwira
● Substrate A 7ml …………………………………………………. .....................................kapu yoyera
● Substrate B7ml ……………………………………………… .....................…..kapu yofiyira
● Stop solution 7ml…………………………………………………………kapu yachikasu
● 20×concentrated wash solution 40ml ……………………….……kapu yowonekera
● 2×concentrated m'zigawo njira 60ml……………………..…………….blue kapu
● 2-Nitrobenzaldehyde 15.1mg…………………………………………………kapu yakuda
4.Sensitivity, kulondola ndi kulondola
Kumverera: 0.025ppb
Malire ozindikira……………………………………………………….0.1ppb
Kulondola:
Minofu ya nyama (minofu ndi chiwindi)…………………….75±15%
Honey……………………………………………………..90±20%
Dzira………………………………………………………..…90±20%
Mkaka………………………………………………………..…90±10%
Kulondola: CV ya zida za ELISA ndi zosakwana 10%.
5.Cross Rate
Furazolidone metabolite (AOZ)……………………………………………..100%
Furaltadone metabolite (AMOZ) ………………………………………<0.1%
Nitrofurantoin metabolite (AHD)……………………………………<0.1%
Nitrofurazone metabolite (SEM)…………………………………………<0.1%
Furazolidone…………………………………………………………….…..… 16.3%
Furaltadone……………………………………………………………….…<1%
Nitrofurantoin ………………………………………………………….…<1%
Nitrofurazone……………………………………………………………..…<1%