Elisa Test Kit ya AOZ
Za
Chidachi chitha kugwiritsidwa ntchito pakuwunika kwachulukidwe komanso koyenera kwa zotsalira za AOZ mu minofu ya nyama (nkhuku, ng'ombe, nkhumba, ndi zina), mkaka, uchi ndi mazira.
Kuwunika kwa zotsalira za mankhwala a nitrofuran kuyenera kutengera kuzindikira kwa minofu yomangidwa ndi metabolites ya mankhwala a makolo a nitrofuran, omwe akuphatikizapo Furazolidone metabolite (AOZ), Furaltadone metabolite (AMOZ), Nitrofurantoin metabolite (AHD) ndi Nitrofurazone metabolite (SEM).
Poyerekeza ndi njira za chromatographic, zida zathu zimawonetsa zabwino zambiri zokhuza kukhudzika, malire ozindikira, zida zamakono komanso nthawi yofunikira.
Zida Zamagetsi
• mbale ya Microtiter yokutidwa ndi antigen, zitsime 96
• Njira zokhazikika (mabotolo 6, 1ml/botolo)
0ppb, 0.025ppb, 0.075ppb, 0.225ppb, 0.675ppb, 2.025ppb
• Spiking control standard : (1ml/botolo)..............................................….100ppb
• Enzyme conjugate concentrate 1.5ml.............................................…….kapu yofiyira
• Antibody solution yakhazikika 0.8ml…………………………………......kapu yobiriwira
• Substrate A 7ml ……………………..........................................…………..kapu yoyera
• Substrate B7ml………………………………...............................................…..kapu yofiyira
• Stop solution 7ml……………………………………………………….………kapu yachikasu
• 20×20xconcentrated wash solution 40ml ……………………….……kapu yowonekera
• 2 × 60ml wothira m'zigawo ………………………..…………….blue cap
• 2-Nitrobenzaldehyde 15.1mg……………………………………………………kapu yakuda
Sensitivity, kulondola ndi kulondola
Kumverera: 0.025ppb
Malire ozindikira…………………………………………………………….1ppb
Kulondola:
Minofu ya nyama (minofu ndi chiwindi)…………………….75±15%
Honey……………………………………………………..90±20%
Dzira………………………………………………………..…90±20%
Mkaka………………………………………………………..…90±10%
Kulondola:CV ya zida za ELISA ndi zosakwana 10%.
Cross Rate
Furazolidone metabolite (AOZ)……………………………………………..100%
Furaltadone metabolite (AMOZ) ………………………………………<0.1%
Nitrofurantoin metabolite (AHD)……………………………………<0.1%
Nitrofurazone metabolite (SEM)…………………………………………<0.1%
Furazolidone…………………………………………………………….…..… 16.3%
Furaltadone……………………………………………………………….…<1%
Nitrofurantoin ………………………………………………………….…<1%
Nitrofurazone……………………………………………………………..…<1%