Elisa Test Kit ya AMOZ
Za
Chidachi chingagwiritsidwe ntchito poyesa kuchuluka komanso kuwunika kwa zotsalira za AMOZ m'madzi am'madzi (nsomba ndi shrimp), ndi zina. Ma enzyme immunoassays, poyerekeza ndi njira za chromatographic, amawonetsa zabwino zambiri zokhuza kukhudzika, malire ozindikira, zida zaukadaulo ndi nthawi yofunikira.
Chidachi chapangidwa kuti chizindikire AMOZ kutengera mfundo ya immunoassay yopikisana ya enzyme.Zitsime za microtiter zidakutidwa ndi Capture BSA yolumikizidwa
antigen.AMOZ mu zitsanzo amapikisana ndi antigen yomwe ili pa mbale ya microtiter ya antibody yomwe yawonjezeredwa.Pambuyo pakuwonjezera kwa enzyme conjugate, gawo lapansi la chromogenic limagwiritsidwa ntchito ndipo chizindikirocho chimayesedwa ndi spectrophotometer.Mayamwidwe ake amafanana mosagwirizana ndi kuchuluka kwa AM OZ pachitsanzo.
Zida Zamagetsi
·Mbale ya Microtiter yokhala ndi zitsime 96 zokutidwa ndi antigen
·Mayankho okhazikika (mabotolo 6)
0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb, 4.05ppb
·Spiking standard solution: (1ml/botolo) …………………………………………………100ppb
· Enzyme conjugate 1ml…………………………………………………………………………….kapu yofiira
· Antibody solution 7ml ………………………………………………………..………….….
Solution A 7ml …………………………………………………………….…………………kapu yoyera
·solution B 7ml………………………………………………………………….………..…… kapu yofiyira
·stop solution 7ml …………………………………………………………….…………kapu yachikasu
·20×20×40ml wash solution ……………………………………….…
·2 × 50ml m'zigawo zitsulo ……………………………………….…….blue cap
·2-Nitrobenzaldehyde 15.1mg…………………………………………………………….
Sensitivity, kulondola ndi kulondola
Kumverera: 0.05ppb
Malire ozindikira
Zam'madzi (nsomba ndi shrimp)……………………… 0.1ppb
Kulondola
Zam'madzi (nsomba ndi shrimp)…………………… 95±25%
Kulondola:CV ya zida za ELISA ndi zosakwana 10%.
Cross Rate
Furaltadone metabolite(AMOZ)…………………………………….…………100%
Furazolidone metabolite(AMOZ)……………………………..………………..<0.1%
Nitrofurantoin metabolite(AHD)………………………………….…………<0.1%
Nitrofurazone metabolite(SEM)……………………………………………....<0.1%
Furaltadone…………………………………………………………………….…….11.1%
Furazolidone …………………………………………………………….……….<0.1%
Nitrofurantoin……………………………………………………………….…<1%
Nitrofurazone……………………………………………………….…………………<1%