Elisa Test Kit ya Aflatoxin B1
Kuchuluka kwa ma aflatoxins kumabweretsa chiphe (aflatoxicosis) chomwe chingakhale chowopseza moyo, nthawi zambiri chifukwa cha kuwonongeka kwa chiwindi.
Aflatoxin B1 ndi aflatoxin yopangidwa ndi Aspergillus flavus ndi A. parasiticus.Ndi carcinogen yamphamvu kwambiri.Mphamvu ya carcinogenic iyi imasiyanasiyana kutengera mitundu ndi ena, monga makoswe ndi anyani, omwe amawoneka kuti ndi owopsa kwambiri kuposa ena.Aflatoxin B1 ndizovuta kwambiri muzakudya zosiyanasiyana kuphatikiza mtedza, ufa wa thonje, chimanga, ndi mbewu zina;komanso zakudya za ziweto.Aflatoxin B1 imatengedwa kuti ndi aflatoxin wapoizoni kwambiri ndipo imakhudzidwa kwambiri ndi kansa ya hepatocellular carcinoma (HCC) mwa anthu.Zitsanzo zingapo ndi njira zowunikira kuphatikiza thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), mass spectrometry, ndi enzyme-linked immunosorbent assay (ELISA), mwa zina, zagwiritsidwa ntchito kuyesa kuipitsidwa kwa aflatoxin B1 muzakudya. .Malinga ndi bungwe la Food and Agriculture Organisation (FAO), kuchuluka kwa aflatoxin B1 padziko lonse lapansi komwe kumaloledwa ndi 1-20 μg/kg m'zakudya, ndi 5-50 μg/kg pazakudya za ng'ombe mu 2003.
Tsatanetsatane
1.Elisa Test Kit ya Aflatoxin B1
2.Mphaka.Zithunzi za KA07202H-96well
3. Zida zamagulu
● Microtiter yokutidwa ndi antigen, zitsime 96
● Standard Solution × 6botolo (1ml/botolo)
0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb
● Enzyme conjugate 7ml……………………………………………………………..…………kapu yofiira
● Antibody solution7ml............................................. ………………………………………………
● Substrate A 7ml…………………………………………………………………………………kapu yoyera
● Substrate B 7ml……………………………………………………………………….…………kapu yofiira
● Stop solution 7ml……….……………………………………………………………..………kapu yachikasu
● 20×concentrated wash solution 40ml ……………………………………………kapu yowonekera
● 2×concentrated extraction solution 50ml…………………………………………………kapu yabuluu
4.Sensitivity, kulondola ndi kulondola
Kumverera: 0.05ppb
5.Kuzindikira malire
Mafuta ophikira amafuta .......................................... .................................................. .......................0.1ppb
Mtedza.................................................................. .................................................. .......................0.2ppb
Cereal................................................. .................................................. .....................0.05ppb
Kulondola
Mafuta ophikira amafuta .......................................... .................................................. .................80±15%
Mtedza.................................................................. .................................................. ....................80±15%
Cereal................................................. .................................................. ....................80±15%
Kulondola:Kusiyanasiyana kwa zida za ELISA ndizochepera 10%.
6.Cross Rate
Aflatoxin B1 ··················100%
Aflatoxin B2 ·······················81 .3%
Aflatoxin G1 ·······················62%
Aflatoxin G2 ·······················22.3%.