Butralin, also known as stopping buds, is a touch and local systemic bud inhibitor, belongs to the low toxicity of dinitroaniline tobacco bud inhibitor, to inhibit the growth of axillary buds of high efficacy, fast efficacy. Butralin test strips apply the principle of competitive inhibition immunochromatography. Butralin extracted from the sample binds to the colloidal gold-labelled specific antibody, which inhibits the binding of the antibody to the butralin-BSA coupler on the T-line of the NC membrane, resulting in a change in the colour of the detection line. When there is no butralin in the sample or the butralin is below the detection limit, the T line shows stronger colour than the C line or there is no difference with the C line; when the butralin in the sample exceeds the detection limit, the T line does not show any colour or it is significantly weaker than the C line; and the C line shows colour regardless of the presence or absence of butralin in the sample to indicate that the test is valid.
Kwinbon's test strips are suitable for the qualitative determination of butralin in tobacco samples (post-harvest to-be-roasted and first-roasted tobacco leaves). Detection limit 5mg/kg, the test shows negative with 10 mg/kg of maleic hydrazide, forchlorfenuron, flumetralin. This video demonstrates the sample pre-treatment process, testing procedure, and result determination.
Post time: Apr-23-2024