Amid the increasingly severe background of food safety issues, a new type of test kit based on the Enzyme-Linked Immunosorbent Assay (ELISA) is gradually becoming an important tool in the field of food safety testing. It not only provides more precise and efficient means for food quality monitoring but also builds a solid defense line for the safety of consumers' food.
The principle of the ELISA test kit lies in utilizing the specific binding reaction between antigen and antibody to quantitatively determine the content of target substances in food through enzyme-catalyzed substrate color development. Its operation process is relatively simple and has high specificity and sensitivity, enabling accurate identification and measurement of harmful substances in food, such as aflatoxin, ochratoxin A, and T-2 toxins.
In terms of specific operational procedures, the ELISA test kit typically includes the following steps:
1. Sample preparation: Firstly, the food sample to be tested needs to be appropriately processed, such as extraction and purification, to obtain a sample solution that can be used for detection.
2. Sample addition: The processed sample solution is added to the designated wells in the ELISA plate, with each well corresponding to a substance to be tested.
3. Incubation: The ELISA plate with added samples is incubated at an appropriate temperature for a period of time to allow full binding between antigens and antibodies.
4. Washing: After the incubation, washing solution is used to remove unbound antigens or antibodies, reducing the interference of nonspecific binding.
5. Substrate addition and color development: Substrate solution is added to each well, and the enzyme on the enzyme-labeled antibody catalyzes the substrate to develop color, forming a colored product.
6. Measurement: The absorbance value of the colored product in each well is measured using instruments such as an ELISA reader. The content of the substance to be tested is then calculated based on a standard curve.
There are numerous application cases of ELISA test kits in food safety testing. For instance, during a routine food safety supervision and sampling inspection, market regulatory authorities utilized an ELISA test kit to rapidly and accurately detect excessive levels of aflatoxin B1 in peanut oil produced by an oil mill. Appropriate penalty measures were promptly taken, effectively preventing the harmful substance from endangering consumers.
Moreover, due to its ease of operation, accuracy, and reliability, the ELISA test kit is widely used in the safety testing of various foods such as aquatic products, meat products, and dairy products. It not only significantly shortens the detection time and improves efficiency but also provides powerful technical support for regulatory authorities to strengthen supervision of the food market.
With the continuous advancement of technology and the increasing awareness of food safety among people, ELISA test kits will play an increasingly important role in the field of food safety testing. In the future, we look forward to the continuous emergence of more technological innovations, jointly promoting the vigorous development of the food safety industry and providing a more solid guarantee for the safety of consumers' food.
Post time: Dec-12-2024