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Whakaahuatanga Poto:

Ka whakakikoruatia te antigen SEM ki te rohe whakamatautau o te kiriuhi nitrocellulose o nga takai, ka tapaina te antibody SEM ki te koura colloid. I te wa o te whakamatautau, ko te koura colloid kua tapaina he antibody kua pania ki roto i te takai ka neke whakamua i te taha o te kiriuhi, ka puta he raina whero ina kohi te antibody me te antigen i te raina whakamatautau; Mena kei runga ake te SEM i roto i te tauira i te rohenga kitenga, ka tauhohe te antigens me nga antigens i roto i te tauira kaore e tutuki i te antigen i te raina whakamatautau, na reira karekau he raina whero i te raina whakamatautau.