Kete Whakamatau Elisa o AOZ
Mō
Ka taea te whakamahi i tenei kete ki te tātari ine me te kounga o te toenga AOZ i roto i nga kopa kararehe (heihei, kau, poaka, aha atu), miraka, honi me nga hua.
Ko te tātaritanga o te toenga o nga raau taero nitrofuran me hangai i runga i te kitenga o nga metabolites herea o te kiko o nga raau matua nitrofuran, tae atu ki te Furazolidone metabolite (AOZ), Furaltadone metabolite (AMOZ), Nitrofurantoin metabolite (AHD) me te Nitrofurazone metabolite (SEM).
Ka whakatauritea ki nga tikanga chromatographic, he nui nga painga o ta maatau kete e pa ana ki te tairongo, te rohe kitenga, nga taputapu hangarau me te waa.
Nga Waahanga Kete
• He pereti Microtiter kua pania ki te antigen, 96 puna
• Nga otinga paerewa(6 ipu,1ml/pounamu)
0ppb,0.025ppb,0.075ppb,0.225ppb,0.675ppb,2.025ppb
• Te mana whakahaere paerewa : (1ml/pounamu)...................................................….100ppb
• Te konukawata conjugate 1.5ml.................................................…..potae whero
• Ko te otinga pareketo 0.8ml ……………………………………....…potae matomato
• Raupapa A 7ml.....................................................................................pee ma
• Raupapa B7ml..................................................................................……..potae whero
• Whakamutua te otinga 7ml…………………………………………………….…… potae kōwhai
• 20×Warewa horoi horoi 40ml ……………………….……puata maramara
• 2×whakarewa tangohanga 60ml…………………………..………….potae kahurangi
• 2-Nitrobenzaldehyde 15.1mg……………………………………………… kapene pango
Te tairongo, te tika me te tika
Te tairongo: 0.025ppb
Tepe rapunga………………………………………………0.1ppb
Tika:
Te kiko kararehe (te uaua me te ate)…………………………75±15%
Honi………………………………..……………………..90±20%
Egg……………………………………………..…90±20%
Miraka………………………………………………………..…90±10%
Tika:Ko te CV o te kete ELISA he iti iho i te 10%.
Reiti Ripeka
Furazolidone metabolite (AOZ)…………………………………………………..100%
Furaltadone metabolite (AMOZ)……………………………………………………<0.1%
Nitrofurantoin metabolite (AHD)……………………………………………………<0.1%
Nitrofurazone metabolite (SEM)……………………………………………………<0.1%
Furazolidone………………………………………………………………..…16.3%
Furaltadone…………………………………………………………………………<1%
Nitrofurantoin……………………………………………………………….…<1%
Nitrofurazone…………………………………………………………………………..…<1%