Kit Tes Elisa saka AOZ
Babagan
Kit iki bisa digunakake ing analisis kuantitatif lan kualitatif residu AOZ ing jaringan kewan (pitik, sapi, babi, lsp), susu, madu lan endhog.
Analisis residu obat nitrofuran kudu adhedhasar deteksi metabolit terikat jaringan saka obat induk nitrofuran, sing kalebu metabolit Furazolidone (AOZ), metabolit Furaltadone (AMOZ), metabolit Nitrofurantoin (AHD) lan metabolit Nitrofurazone (SEM).
Dibandhingake karo metode kromatografi, kit kita nuduhake kaluwihan sing akeh babagan sensitivitas, watesan deteksi, peralatan teknis lan syarat wektu.
Komponen Kit
• piring Microtiter ditutupi karo antigen, 96 sumur
• Solusi standar (6 botol, 1ml/botol)
0ppb, 0,025ppb, 0,075ppb, 0,225ppb, 0,675ppb, 2,025ppb
• Kontrol standar Spiking : (1ml/botol)...................................................….100ppb
• Konsentrat konjugasi enzim 1,5ml.................................................….. tutup abang
• Larutan antibodi konsentrat 0,8ml………………………………...…topi ijo
• Substrat A 7ml.........................................................................................topi putih
• Substrat B7ml………………………………………….........................................……….. tutup abang
• Stop solution 7ml………………………………………….……. tutup kuning
• 20×solusi cuci konsentrat 40ml …………………………………………. tutup transparan
• 2×larutan ekstraksi pekat 60ml……………………..……………….topi biru
• 2-Nitrobenzaldehyde 15.1mg…………………………………………………………………………………………………………………………………………………………………………………………
Sensitivitas, akurasi lan presisi
Sensitivitas: 0.025ppb
watesan deteksi…………………………………………… 0.1ppb
Akurasi:
Jaringan kewan (otot lan ati)………………………… 75±15%
Madu……………………………………………………..90±20%
Endhog ……………………………………………………..90±20%
Susu ……………………………………………………..90±10%
Presisi:CV kit ELISA kurang saka 10%.
Cross Rate
Metabolit Furazolidone (AOZ) ……………………………………………..100%
Metabolit Furaltadone (AMOZ)……………………………………………………<0.1%
Metabolit Nitrofurantoin (AHD)…………………………………………<0,1%
Metabolit Nitrofurazon (SEM)……………………………………………………<0.1%
Furazolidone …………………………………………………………………… 16,3%
Furaltadone ……………………………………………………………………<1%
Nitrofurantoin ………………………………………………………….…<1%
Nitrofurazone ……………………………………………………………………..<1%