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Dosis aflatoksin sing akeh nyebabake keracunan akut (aflatoksikosis) sing bisa ngancam nyawa, biasane amarga karusakan ing ati.
Aflatoksin B1 yaiku aflatoksin sing diprodhuksi dening Aspergillus flavus lan A. parasiticus.Iku karsinogen banget kuat.Potensi karsinogenik iki beda-beda ing antarane spesies karo sawetara, kayata tikus lan kethek, katon luwih rentan tinimbang liyane.Aflatoksin B1 minangka contaminant umum ing macem-macem panganan kalebu kacang, meal cottonseed, jagung, lan biji-bijian liyane;uga pakan kewan.Aflatoksin B1 dianggep minangka aflatoksin paling beracun lan kena pengaruh banget ing karsinoma hepatoseluler (HCC) ing manungsa.Sawetara cara sampling lan analitis kalebu kromatografi lapisan tipis (TLC), kromatografi cair kinerja dhuwur (HPLC), spektrometri massa, lan uji immunosorbent enzim-linked (ELISA), antara liya, wis digunakake kanggo nguji kontaminasi aflatoksin B1 ing panganan. .Miturut Organisasi Pangan lan Pertanian (FAO), tingkat maksimum aflatoksin B1 sing ditoleransi ing saindenging jagad dilaporake ana ing kisaran 1-20 μg / kg ing panganan, lan 5-50 μg / kg ing pakan ternak ing taun 2003.

Rincian

1. Elisa Test Kit kanggo Aflatoksin B1

2. Kucing.Sumur KA07202H-96

3. komponen Kit
● Microtiter plateprecoated karo antigen, 96 sumur
● Solusi Standar ×6botol(1ml/botol)
0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb
● Konjugat enzim 7ml………………………………………………………………………………………………
● Solusi antibodi 7ml................................................................ .................................................. tutup ijo
● Substrat A 7ml ………………………………………………………………………………………… tutup putih
● Substrat B 7ml……………………………………………………………………………………. tutup abang
● Stop solution 7ml……………………………………………………………………………………………….. tutup kuning
● 20×solusi cuci konsentrat 40ml …………………………………………tutup transparan
● 2×larutan ekstraksi pekat 50ml………………………………………………tutup biru

4.Sensitivitas, akurasi lan presisi
Sensitivitas: 0.05ppb

5. Watesan deteksi
Sampel minyak goreng................................................................ ................................................... ........................0.1ppb
Kacang................................................. ................................................... .......................0.2ppb
Sereal ................................................. ................................................... ...................0.05ppb
Akurasi
Sampel minyak goreng................................................................ ................................................... ....................80±15%
Kacang................................................. ................................................... ...................80±15%
Sereal ................................................. ................................................... ...................80±15%
Presisi:Koefisien variasi kit ELISA kurang saka 10%.

6. Nilai silang
Aflatoksin B1················ 100%
Aflatoksin B2························ 81 ,3%
Aflatoksin G1······················· 62%
Aflatoksin G2······················· 22,3%