China Immunoaffinity ogidi maka ụlọ ọrụ nchọpụta Zearalenone na ndị na-emepụta

Ogidi mgbochi ọrịa maka nchọpụta Zearalenone Onyonyo agbapụtara

Ogidi mgbochi mgbochi maka nchọpụta Zearalenone

Nkọwa dị mkpirikpi:

A na-eji kọlụm Kwinbon Zearalenone site na ijikọta ya na ngwa ule HPLC, LC-MS, ELISA. Ọ nwere ike ịbụ ule ọnụọgụ AFB1 maka ọka na ngwaahịa ọka, soy sauce, mmanya mmanya, ngwaahịa ihendori, mmanya na-aba n'anya, soybean, rapeseed na mmanụ ihe oriri, nri akụrụngwa na ngwaahịa emechara, wdg.

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Nkọwa ngwaahịa

Mkpado ngwaahịa

Nkọwapụta ngwaahịa

Nwamba mba.	KH00304Z
Njirimara	MakaZearalenoneule
Ebe Mmalite	Beijing, China
Aha ika	Kwinbon
Nha nkeji	25 ule kwa igbe
Ngwa nlele	Ọka na ngwaahịa ọka, soy sauce, mmanya mmanya, ngwaahịa ihendori, mmanya na-aba n'anya, soybean, mkpụrụ rapeseed na mmanụ nri, nri akụrụngwa na ngwaahịa emechara, wdg.
Nchekwa	2-30 ℃
Ndụ nchekwa	Ọnwa iri na abụọ
Nnyefe	Okpomọkụ ụlọ

Akụrụngwa & Reagents achọrọ

Akụrụngwa

Reagents

Akụrụngwa

----Homogenizer ---- igwekota Vortex

----Sample karama ---- Ịtụ cylinder: 10ml, 100ml

---- Akwụkwọ nzacha qualitative/Centrifuge ---- Ntụle nyocha (inductance: 0.01g)

----Piette gụchara: 10ml ----Injector: 20ml

---- Mpempe ụda olu: 250ml ---- bọlbụ pipette roba

----Micropipette: 100-1000ul ----Glass funnel 50ml

----Microfiber Filters (Whatman, 934-AH, Φ11cm, 1.5um okirikiri)

Reagents

----Methanol (AR)

----Acetic acid (AR)

----Sodium chloride (NACL, AR)

----Mmiri a na-agbapụta

Uru ngwaahịa

Zearalenone (ZEN), nke a makwaara dị ka RAL na F-2 mycotoxin, bụ metabolite estrogenic dị ike mepụtara. Ọ na-ekpo ọkụ na-ekpo ọkụ, a na-ahụkwa ya n'ụwa nile n'ọtụtụ mkpụrụ ọka, dị ka ọka, ọka bali, ọka, ọka wit, osikapa, na sorghum.

Zearalenone bụ isi nsí nke na-ejikọta na ndị na-anabata estrogen, na-akpata infertility, ite ime ma ọ bụ nsogbu ọmụmụ ndị ọzọ, karịsịa na ezì.

Wikipedia na-akwado ụzọ nchọpụta ndị a;

chromatography nke oyi akwa,
Enzyme-njikọ immunosorbent nyocha
Fluorescence kọlụm immunoaffinity
Immunoaffinity kọlụm dị elu arụmọrụ chromatography mmiri mmiri

Ogidi Kwinbon Inmmunoaffinity bụ usoro nke atọ, ọ na-eji chromatography mmiri mmiri maka nkewa, ịdị ọcha ma ọ bụ nyocha kpọmkwem nke Zearalenone. A na-ejikọta kọlụm Kwinbon na HPLC.

HPLC quantitative analysis of fungal toxin bụ usoro nchọpụta tozuru okè. Ma chromatography na-aga n'ihu na nke azụ ka ọdabara. Azụ azụ HPLC bụ nke akụ na ụba, dị mfe iji rụọ ọrụ ma nwee nsi mgbaze dị ala. Ọtụtụ nsí na-agbaze na usoro mkpanaka polar wee kewapụ ya site na kọlụm chromatography na-abụghị polar, na-egbo mkpa maka ịchọpụta ngwa ngwa nke ọtụtụ nsị fungal na nlele mmiri ara ehi. A na-eji nwayọọ nwayọọ na-etinye ihe nchọpụta jikọtara UPLC, yana modul nrụgide dị elu na obere nha na ogidi chromatography nke dị ntakịrị, nke nwere ike belata oge ịgba ọsọ, melite arụmọrụ nkewa chromatographic, ma nweta mmetụta dị elu.

Site na nkọwapụta dị elu, kọlụm Kwinbon Zearalenone nwere ike ijide ụmụ irighiri ihe ezubere iche na steeti dị ọcha nke ukwuu. Ọzọkwa kọlụm Kwinbon na-agba ọsọ ọsọ, dị mfe iji rụọ ọrụ. Ugbu a, a na-eji ngwa ngwa na ebe niile na nri na ubi ọka maka aghụghọ mycotoxins.