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Ngwa ule Elisa nke Ochratoxin A gosipụtara onyonyo
  • Ngwa ule Elisa nke Ochratoxin A
  • Ngwa ule Elisa nke Ochratoxin A

Ngwa ule Elisa nke Ochratoxin A

Nkọwa dị mkpirikpi:

Enwere ike iji ngwa a na nyocha ọnụọgụ na qualitative nke ochratoxin A na nri.Ọ bụ ngwaahịa ọhụrụ maka nchọpụta ihe fọdụrụ ọgwụ dabere na teknụzụ ELISA, nke na-efu naanị 30min n'ọrụ ọ bụla ma nwee ike ibelata mperi ọrụ yana ike ọrụ.Ngwa a dabere na teknụzụ ELISA asọmpi na-apụtaghị ìhè.A na-ekpuchi olulu mmiri microtiter na antigen njikọ.Ochratoxin A na sample na-asọ mpi na antigen mkpuchi na efere microtiter maka ntibody agbakwunyere.Mgbe mgbakwunye nke enzyme conjugate, a na-eji mkpụrụ TMB gosi agba.Mwepu nke sample bụ ihe na-adịghị mma metụtara o chratoxin A n'ime ya, ka e jiri ya tụnyere Standard Curve, mụbaa site dilution ihe, Ochratoxin A quantity na sample nwere ike gbakọọ.


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Nkọwa ngwaahịa

Mkpado ngwaahịa

Ochratoxins bụ otu mycotoxins nke ụfọdụ ụdị Aspergillus mepụtara (karịsịa A).Ochratoxin A mara na ọ na-eme na ngwa ahịa dị ka ọka, kọfị, mkpụrụ osisi mịrị amị na mmanya uhie.A na-ewere ya dị ka carcinogen mmadụ ma nwee mmasị pụrụ iche dịka enwere ike ịkwakọba ya na anụ anụmanụ.Ya mere, anụ na ngwaahịa anụ nwere ike iji nsị a metọọ.Ikpughe na ochratoxins site na nri nwere ike inwe oke nsi na akụrụ mammalian, ma nwee ike bụrụ carcinogenic.

Nkọwa

1. Ngwa ule Elisa nke Ochratoxin A

2. Nwamba.KA07301H-96 olulu mmiri

3. Ngwa ngwa
● Microtiter efere nwere olulu mmiri 96 nke ejiri antigen kpuchie
● Standard ngwọta (6 karama: 1ml / karama)
0ppb, 0.4ppb, 0.8ppb, 1.6ppb, 3.2ppb, 6.4ppb
● Enzyme conjugate 7ml……………………………………………………………………………………………………….. okpu uhie.
● Ihe mgbochi mgbochi 10ml………………………………………………………………………………………….…. okpu akwụkwọ ndụ akwụkwọ ndụ
● ngwọta mkpụrụ A 7ml………………………………………………………………………………………………………………………………………
● Ngwọta mkpụrụ osisi B 7ml…………………………………………………………………………………………………………………
● Kwụsị ngwọta 7ml ……………………………………………………………………………………………………
● 20× concentrated wash ngwọta 40ml…………………………………………………………………………………………. okpu transperent

4. Mmetụta, izi ezi na nkenke
Nleba anya ule: 0.4ppb
Oke nchọpụta
Nri………………………………………………………………………………………………………………………….5ppb
izi ezi
Nri ...................................................... ... 90 ± 20%
Nkenkenke:Ọnụego mgbanwe nke ngwa ELISA erughị 10%.

5. Ọnụego gafere
Ochratoxin A………………………………………………………………………………………………………………………………………………………………………….

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