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Ngwa ule Elisa nke Ochratoxin A gosipụtara onyonyo
  • Ngwa ule Elisa nke Ochratoxin A
  • Ngwa ule Elisa nke Ochratoxin A
  • Ngwa ule Elisa nke Ochratoxin A
  • Ngwa ule Elisa nke Ochratoxin A

Ngwa ule Elisa nke Ochratoxin A

Nkọwa dị mkpirikpi:

Ochratoxins bụ otu mycotoxins nke ụfọdụ ụdị Aspergillus mepụtara (karịsịa A).Ochratoxin A mara na ọ na-eme na ngwa ahịa dị ka ọka, kọfị, mkpụrụ osisi mịrị amị na mmanya uhie.A na-ewere ya dị ka carcinogen mmadụ ma nwee mmasị pụrụ iche dịka enwere ike ịkwakọba ya na anụ anụmanụ.Ya mere, anụ na ngwaahịa anụ nwere ike iji nsị a metọọ.Ikpughe na ochratoxins site na nri nwere ike inwe oke nsi na akụrụ mammalian, ma nwee ike bụrụ carcinogenic.


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Nkọwa ngwaahịa

Mkpado ngwaahịa

Ihe gbasara

Enwere ike iji ngwa a na nyocha ọnụọgụ na qualitative nke ochratoxin A na nri.Ọ bụ ngwaahịa ọhụrụ maka nchọpụta ihe fọdụrụ ọgwụ dabere na teknụzụ ELISA, nke na-efu naanị 30min n'ọrụ ọ bụla ma nwee ike ibelata mperi ọrụ yana ike ọrụ.Ngwa a dabere na teknụzụ ELISA asọmpi na-apụtaghị ìhè.A na-ekpuchi olulu mmiri microtiter na antigen njikọ.Ochratoxin A na sample na-asọ mpi na antigen mkpuchi na efere microtiter maka ntibody agbakwunyere.Mgbe mgbakwunye nke enzyme conjugate, a na-eji mkpụrụ TMB gosi agba.Mwepu nke ihe nlele ahụ na-emetụtaghị o chratoxin A n'ime ya, mgbe atụnyere ya na Standard Curve, mụbaa site na ihe dilution,OEnwere ike ịgbakọ ọnụọgụ chratoxin n'ime nlele ahụ.

Ngwa ngwa

• Microtiter efere nwere olulu mmiri 96 nke ejiri antigen kpuchie ya

•Standard ngwọta (6 karama: 1ml / karama)

0ppb, 0.4ppb, 0.8ppb, 1.6ppb, 3.2ppb, 6.4ppb

• Enzymeconjugate7ml …………………………………………………………………………………………..…………..…..okpu uhie

• ngwọta mgbochi10ml …………………………………………………………………………………………...….… okpu akwụkwọ ndụ akwụkwọ ndụ

•Mkpụrụ solu A 7ml………………………………………………………………………………………………………………………………………………………………………

•MkpụrụNgwọta B 7ml………………………………………………………………………………………..…………………………. okpu uhie

• Nkwụsị ngwọta 7ml ………………………………………………………………………………………….…………………………. Okpu edo edo

• 20 × lekwasịrị anya saa ngwọta 40ml………...………………………………………….…...… okpu transperent

Mmetụta, izi ezi na nkenke

Nleba anya ule: 0.4ppb

Oke nchọpụta

Nri……………………………………………………………….………………………………………………… 5ppb

izi ezi

Nri……………………………………………………………………………….………………… 90±20%

Nkenkenke

Ọnụego mgbanwe nke ngwa ELISA erughị 10%.

Ọnụọgụ gafere

Ochratoxin A………………………………………………………………..………………………………………………….

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