Ngwa ELisa Test nke AOZ
Ihe gbasara
Enwere ike iji ngwa a mee ihe na nyocha nke ọnụọgụ na nyocha nke AOZ fọdụrụnụ na anụ anụ anụ (ọkụkọ, ehi, ezi, wdg), mmiri ara ehi , mmanụ aṅụ na akwa.
Nyocha nke ihe fọdụrụ na ọgwụ nitrofuran kwesịrị ịdabere na nchọpụta nke anụ ahụ na-ejikọta metabolites nke ọgwụ nne na nna nitrofuran, nke gụnyere Furazolidone metabolite (AOZ), Furaltadone metabolite (AMOZ), Nitrofurantoin metabolite (AHD) na Nitrofurazone metabolite (SEM).
Tụnyere usoro chromatographic, ngwa anyị na-egosi uru dị ukwuu gbasara uche, oke nchọpụta, akụrụngwa teknụzụ na oge achọrọ.
Ngwa ngwa
• Efere Microtiter kpuchie na antigen, olulu mmiri 96
• Standard ngwọta (6 karama, 1ml / karama)
0ppb,0.025ppb,0.075ppb,0.225ppb,0.675ppb,2.025ppb
• Spiking ọkọlọtọ njikwa: (1ml / karama).....................................................….100pb
• Enzyme conjugate itinye uche 1.5ml ....................................................……. okpu uhie
• Ihe ngwọta mgbochi gbadoro anya 0.8ml………………………………………………....… okpu akwụkwọ ndụ akwụkwọ ndụ
• Mkpụrụ A 7ml……………………………………….................................................………………………. okpu ọcha
• Mgbochi B7ml………………………………………………......................................................... okpu uhie
• Nkwụsị ngwọta 7ml……………………………………………………………………….……… okpu edo edo
• 20 × lekwasịrị anya asacha ngwọta 40ml …………………………………………. transperent okpu
• 2 × lekwasịrị anya mmịpụta ngwọta 60ml………………………………...…………………. okpu na-acha anụnụ anụnụ
• 2-Nitrobenzaldehyde 15.1mg………………………………………………………………………………….
Mmetụta, izi ezi na nkenke
Mmetụta: 0.025pb
Oke nchọpụta…………………………………………………………………………… 0.1 pb
izi ezi:
anụ ahụ anụ (ahụ na imeju)……………………………………… 75± 15%
Mmanụ aṅụ……………………………………………………………………………………………………….90±20%
Àkwá ................................................... .. ... 90 ± 20%
Mmiri ara ehi……………………………………………………………………………………………………………… 90±10%
Nkenkenke:CV nke ngwa ELISA erughị 10%.
Ọnụọgụ gafere
Furazolidone metabolite (AOZ) …………………………………………………………………………………………
Furaltadone metabolite (AMOZ) …………………………………………………………………………
Nitrofurantoin metabolite (AHD) …………………………………………………………………………
Nitrofurazone metabolite (SEM) …………………………………………………………………………………………….
Furazolidone …………………………………………………………………………………………………………………………
Amegbu :............................................... ... <1%
Nitrofurantoin………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………….
Nitrofursone ................................................ ... <1%