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Ngwa ule Elisa nke onyonyo egosipụtara AMOZ
  • Ngwa ule Elisa nke AMOZ
  • Ngwa ule Elisa nke AMOZ

Ngwa ule Elisa nke AMOZ

Nkọwa dị mkpirikpi:

Enwere ike iji ngwa a mee ihe na nyocha nke quantitative na qualitative nke AMOZ fọdụrụnụ na ngwaahịa mmiri (azụ na oporo), wdg Enzyme immunoassays, ma e jiri ya tụnyere usoro chromatographic, na-egosi uru dị ukwuu banyere mmetụta uche, njedebe nchọpụta, akụrụngwa teknụzụ na oge achọrọ.
Emebere ngwa a iji chọpụta AMOZ dabere na ụkpụrụ nke immunoassay enzyme asọmpi na-apụtaghị ìhè.A na-ejikọta olulu mmiri microtiter na njide BSA
antigen.AMOZ na sample na-asọmpi na antigen mkpuchi na efere microtiter maka mgbochi agbakwunyere.Mgbe mgbakwunye nke enzyme conjugate, a na-eji mkpụrụ chromogenic eme ihe wee tụọ mgbama site na spectrophotometer.Mmụba a na-agbanwe agbanwe na ntinye AM OZ na nlele.


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Nkọwa ngwaahịa

Mkpado ngwaahịa

2.The nitrofuran ọgwụ furaltadone, nitrofurantoin na nitrofurazone ka amachibidoro iji na-emepụta anụmanụ nri na EU na 1993, na iji furazolidone na-amachibidoro na 1995. Nyocha nke nitrofuran ọgwụ fọdụrụnụ kwesịrị ịdabere na nchọpụta nke anụ ahụ. ejikọta metabolites nke ọgwụ nne na nna nitrofuran, ebe ọ bụ na ọgwụ ndị nne na nna na-emewanye ngwa ngwa, na anụ ahụ nke nitrofuran metabolites ga-ejigide ogologo oge, ya mere a na-eji metabolites mee ihe dị ka ihe mgbaru ọsọ na nchọpụta nke mmetọ nke nitrofurans.Furazolidone metabolite (AMOZ), Furaltadone metabolite (AMOZ), Nitrofurantoin metabolite (AHD) na Nitrofurazone metabolite (SEM).

Nkọwa

1.Elisa Test Kit nke AMOZ

2.Nwamba.KA00205H-96 olulu mmiri

3. Ngwa ngwa
● Microtiter efere nwere olulu mmiri 96 nke ejiri antigen kpuchie
● Ngwọta ọkọlọtọ ( karama 6)
0ppb, 0.05ppb,0.15ppb,0.45ppb,1.35ppb,4.05ppb
● Ngwọta ọkọlọtọ: (1ml/ karama) …………………………………………………………………….100ppb
● Enzyme conjugate 1ml…………………………………………………………………………………. okpu uhie
● Ihe mgbochi mgbochi 7ml ……………………………………………………………………………. okpu akwụkwọ ndụ akwụkwọ ndụ
● ngwọta A 7ml…………………………………………………………………………………………………………………………………
● ngwọta B 7ml…………………………………………………………………………………………………………. okpu uhie
● ngwọta nkwụsị 7ml …………………………………………………………………………………
● 20 × lekwasịrị asacha ngwọta 40ml……………………………………………………………. transperent okpu.
● Ihe ngwọta mmịpụta 2 × 50ml……………………………………………………………………………….. okpu na-acha anụnụ anụnụ
● 2-Nitrobenzaldehyde 15.1mg………………………………………………………………………………………….

4.Sensitivity, izi ezi na nkenke
Mmetụta: 0.05ppb
Oke nchọpụta
Ngwaahịa mmiri (azụ na oporo)……………………………… 0.1ppb
izi ezi
Ngwaahịa mmiri (azụ na oporo) ………………………………… 95± 25%
Nkenke: CV nke ngwa ELISA erughị 10%.

5.Cross Ọnụego
Furaltadone metabolite (AMOZ) ……………………………………………………………………………………………………………………
Furazolidone metabolite (AMOZ) ………………………………………………………………………………………….
Nitrofurantoin metabolite (AHD) …………………………………………………………
Nitrofurazone metabolite (SEM)………………………………………………………………………………………………………………………………………………
Furaltadone ………………………………………………………………………………………………………………………………….11.1%
Rurazolidone .................................................................................. ... <0.1%
Nitrofurantoin………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………….
Nitrofurazone ………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………….

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