ngwaahịa

Nnukwu doses nke aflatoxins na-eduga na nnukwu nsị (aflatoxicosis) nke nwere ike ịdị egwu ndụ, na-emekarị site na mmebi imeju.
Aflatoxin B1 bụ aflatoxin nke Aspergillus flavus na A. parasiticus mepụtara.Ọ bụ carcinogen dị ike nke ukwuu.Ike carcinogenic a dịgasị iche iche n'ụdị dị iche iche na ụfọdụ, dị ka oke na enwe, yiri ka ọ dị mfe karịa ndị ọzọ.Aflatoxin B1 bụ ihe na-efe efe n'ụdị nri dị iche iche gụnyere ahụekere, nri mkpụrụ owu, ọka, na ọka ndị ọzọ;yana nri anụmanụ.A na-ewere Aflatoxin B1 dị ka aflatoxin kacha egbu egbu na ọ na-etinyekwa ya nke ukwuu na ọrịa hepatocellular carcinoma (HCC) na ụmụ mmadụ.Ọtụtụ usoro nlele na nyocha gụnyere chromatography thin-layer (TLC), chromatography mmiri na-arụ ọrụ dị elu (HPLC), spectrometry mass spectrometry, na enzyme-linked immunosorbent assay (ELISA), n'etiti ndị ọzọ, ejirila nwalee maka mmetọ aflatoxin B1 na nri. .Dị ka Òtù Na-ahụ Maka Nri na Ọrụ Ugbo (FAO) si kwuo, a kọrọ na aflatoxin B1 kasị anabatara n'ụwa nile dị na 1-20 μg/kg n'ihe oriri, na 5-50 μg/kg na nri ehi na-eri nri na 2003.

Nkọwa

1.Elisa Test Kit maka Aflatoxin B1

2.Nwamba.KA07202H-96 olulu mmiri

3. Ngwa ngwa
● Microtiter platecoated na antigen, olulu mmiri 96
● Ọkọlọtọ Ngwọta ×6 bottle(1ml/kalama)
0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb
● Enzyme conjugate 7ml…………………………………………………………………………………………………
● Antibody solution7ml............................................ ................................................Okpu akwụkwọ ndụ akwụkwọ ndụ
● Mkpụrụ A 7ml………………………………………………………………………………………………………………………
● Mkpụrụ B 7ml…………………………………………………………………………………………………………………………
● Kwụsị ngwọta 7ml………………………………………………………………………………………………………………………………
● 20 × lekwasịrị anya asacha ngwọta 40ml …………………………………………………………………………………………………………
● Ihe ngwọta mmịpụta 2 × 50ml………………………………………………………………………………………………………………

4.Sensitivity, izi ezi na nkenke
Mmetụta: 0.05ppb

5.Nchọpụta njedebe
Nlere mmanụ oriri.................................................. ................................................. .........................0.1pb
Ahụekere................................................. ................................................. ......................0.2 ppb
Cereal................................................. ................................................. ..................0.05ppb
izi ezi
Nlere mmanụ oriri.................................................. ................................................. ...................80±15%
Ahụekere................................................. ................................................. ......................80±15%
Cereal................................................. ................................................. ......................80±15%
Nkenkenke:Ọnụego mgbanwe nke ngwa ELISA erughị 10%.

6.Cross Ọnụego
Aflatoxin B1.
Aflatoxin B2······························································································81 .3%
Aflatoxin G1························································ 62%
Aflatoxin G2···································································································22.3%