Whatsapp:+8613811888134

ngwaahịa

Ngwa ule Elisa nke Aflatoxin B1 egosipụtara
  • Ngwa ule Elisa nke Aflatoxin B1
  • Ngwa ule Elisa nke Aflatoxin B1
  • Ngwa ule Elisa nke Aflatoxin B1

Ngwa ule Elisa nke Aflatoxin B1

Nkọwa dị mkpirikpi:

Nnukwu doses nke aflatoxins na-eduga na nnukwu nsị (aflatoxicosis) nke nwere ike ịdị egwu ndụ, na-emekarị site na mmebi imeju.

Aflatoxin B1 bụ aflatoxin nke Aspergillus flavus na A. parasiticus mepụtara.Ọ bụ carcinogen dị ike nke ukwuu.Ike carcinogenic a dịgasị iche iche n'ụdị dị iche iche na ụfọdụ, dị ka oke na enwe, yiri ka ọ dị mfe karịa ndị ọzọ.Aflatoxin B1 bụ ihe na-efe efe n'ụdị nri dị iche iche gụnyere ahụekere, nri mkpụrụ owu, ọka, na ọka ndị ọzọ;yana nri anụmanụ.A na-ewere Aflatoxin B1 dị ka aflatoxin kacha egbu egbu na ọ na-etinyekwa ya nke ukwuu na ọrịa hepatocellular carcinoma (HCC) na ụmụ mmadụ.Ọtụtụ usoro nlele na nyocha gụnyere chromatography thin-layer (TLC), chromatography mmiri na-arụ ọrụ dị elu (HPLC), spectrometry mass spectrometry, na enzyme-linked immunosorbent assay (ELISA), n'etiti ndị ọzọ, ejirila nwalee maka mmetọ aflatoxin B1 na nri. .Dị ka Òtù Na-ahụ Maka Nri na Ọrụ Ugbo (FAO) si kwuo, a kọrọ na aflatoxin B1 kasị anabatara n'ụwa nile dị na 1-20 μg/kg n'ihe oriri, na 5-50 μg/kg na nri ehi na-eri nri na 2003.


Ntuziaka maka ojiji

Nkọwa ngwaahịa

Mkpado ngwaahịa

Ihe gbasara

Enwere ike iji ngwa a maka nyocha qualitative na quantitative nke aflatoxin B1 na mmanụ oriri, ahụekere, ọka cereal, soy sauce, mmanya na nri (nri nri, ihe ngwakọta agwakọta na ihe ndị etinyere).. nyocha ngwá ọrụ.

Ngwaahịa a dabere na ELISA asọmpi na-apụtaghị ìhè, nke dị ngwa ngwa, nke ziri ezi ma nwee mmetụta ma e jiri ya tụnyere nyocha ngwa ngwa.Ọ chọrọ naanị 45min n'otu ọrụ, nke nwere ike ibelata njehie ọrụ yana ike ọrụ.

Ngwa ngwa

• Microtiter platecoated na antigen, olulu mmiri 96

• Ọkọlọtọ Ngwọta ×6 bottle(1ml/kalama)

0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb

• Enzyme conjugate 7ml…………………………………………………………………………………………………………

• Antibody solution7ml............................................................ .................... okpu akwụkwọ ndụ akwụkwọ ndụ

• Mkpụrụ A 7ml……………………………………………………………………………….…………………………. okpu ọcha

• Mkpụrụ B 7ml……………………………………………………………………………………………………….…………. okpu uhie

• Nkwụsị ngwọta 7ml………………………………………………………………………………………………..........Okpu edo edo

• 20 × lekwasịrị anya asacha ngwọta 40ml ……………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………

• Ihe ngwọta mmịpụta 2 × 50ml………………………………………………………………………………………………………………

Mmetụta, izi ezi na nkenke

Mmetụta:0.05pb

Oke nchọpụta

Nlere mmanụ oriri.................................................. .................................................................................0.1pb

Ahụekere................................................. ................................................. ......................0.2 ppb

Cereal................................................. ................................................. ..................0.05ppb

izi ezi

Nlere mmanụ oriri.................................................. ..........................................................................80±15%

Ahụekere................................................. ................................................. ......................80±15%

Cereal................................................. ................................................. ......................80±15%

Nkenkenke

Ọnụego mgbanwe nke ngwa ELISA erughị 10%.

Ọnụọgụ gafere

Aflatoxin B1·····································100%

Aflatoxin B2···························································································81 .3%

Aflatoxin G1··········································································································································································································································.%

Aflatoxin G2.%

  • Nke gara aga:
  • Osote:
    • Dee ozi gị ebe a ziga anyị ya

    ngwaahịa ndị metụtara ya