Elisa Tès Twous Aflatoxin B1
Gwo dòz aflatoksin mennen nan anpwazònman egi (aflatoksikoz) ki ka menase lavi, anjeneral nan domaj nan fwa a.
Aflatoxin B1 se yon aflatoksin ki pwodui pa Aspergillus flavus ak A. parasiticus.Li se yon kanserojèn trè pisan.Pisans kanserojèn sa a varye atravè espès ak kèk, tankou rat ak makak, w pèdi pi sansib pase lòt.Aflatoxin B1 se yon kontaminan komen nan yon varyete manje ki gen ladan pistach, repa grenn koton, mayi, ak lòt grenn;osi byen ke manje bèt.Aflatoxin B1 konsidere kòm aflatoxin ki pi toksik epi li trè enplike nan karsinom epatoselilè (HCC) nan imen. [sitasyon bezwen] Nan bèt, aflatoxin B1 te montre tou yo dwe mutagenic, teratogenic, ak lakòz imunosuppresyon.Plizyè echantiyon ak metòd analyse ki gen ladan chromatografi kouch mens (TLC), segondè-pèfòmans chromatografi likid (HPLC), espektrometri mas, ak tès imunosorban ki lye ak anzim (ELISA), pami lòt moun, yo te itilize pou teste kontaminasyon aflatoxin B1 nan manje. .Dapre Òganizasyon Manje ak Agrikilti (FAO), nivo maksimòm aflatoksin B1 tolere atravè lemond yo te nan ranje 1-20 μg / kg nan manje, ak 5-50 μg / kg nan manje bèt dyetetik nan 2003.
Detay yo
1.Elisa Tès Twous pou Aflatoxin B1
2.Cat.KA07202H-96wells
3. Kit konpozan
● Microtiter plateprecoated ak antijèn, 96 pwi
● Solisyon Creole × 6bottle (1ml / boutèy)
0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb
● Anzim konjige 7ml……………………………………………………..………bouchon wouj
● Antikò solisyon7ml.................................................. ...................................................……bouchon vèt
● Substra A 7ml………………………………………………………….………...…bouchon blan
● Substra B 7ml……………………………………………………………………….………bouchon wouj
● Stop Stop @ estope solisyon 7ml……….…….……………………………………………………..………bouchon jòn
● 20×konsantre solisyon lave 40ml ………………………………………… bouchon transparan
● 2×konsantre solisyon ekstraksyon 50ml……………………………………………………… ble bouchon
4.Sensitivity, presizyon ak presizyon
Sansiblite: 0.05ppb
5.Deteksyon limit
Echantiyon lwil manjab.................................................. .................................................. ........................0.1ppb
Pistach .................................................. .................................................. .......................0.2ppb
Sereyal................................................. .................................................. ......................0.05ppb
Presizyon
Echantiyon lwil manjab.................................................. .................................................. ..................80±15%
Pistach .................................................. .................................................. .....................80±15%
Sereyal................................................. .................................................. .....................80±15%
Presizyon:Varyasyon koyefisyan nan twous ELISA a se mwens pase 10%.
6.Cross Pousantaj
Aflatoxin B1·················100%
Aflatoxin B2······················81 .3%
Aflatoxin G1·······················62%
Aflatoxin G2·······················22.3%