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Twous Tès Elisa nan Aflatoxin B1 En Imaj
  • Elisa Tès Twous Aflatoxin B1
  • Elisa Tès Twous Aflatoxin B1
  • Elisa Tès Twous Aflatoxin B1

Elisa Tès Twous Aflatoxin B1

Deskripsyon kout:

Gwo dòz aflatoksin mennen nan anpwazònman egi (aflatoksikoz) ki ka menase lavi, anjeneral nan domaj nan fwa a.

Aflatoxin B1 se yon aflatoksin ki pwodui pa Aspergillus flavus ak A. parasiticus.Li se yon kanserojèn trè pisan.Pisans kanserojèn sa a varye atravè espès ak kèk, tankou rat ak makak, w pèdi pi sansib pase lòt.Aflatoxin B1 se yon kontaminan komen nan yon varyete manje ki gen ladan pistach, repa grenn koton, mayi, ak lòt grenn;osi byen ke manje bèt.Aflatoxin B1 konsidere kòm aflatoxin ki pi toksik epi li trè enplike nan karsinom epatoselilè (HCC) nan imen. [sitasyon bezwen] Nan bèt, aflatoxin B1 te montre tou yo dwe mutagenic, teratogenic, ak lakòz imunosuppresyon.Plizyè echantiyon ak metòd analyse ki gen ladan chromatografi kouch mens (TLC), segondè-pèfòmans chromatografi likid (HPLC), espektrometri mas, ak tès imunosorban ki lye ak anzim (ELISA), pami lòt moun, yo te itilize pou teste kontaminasyon aflatoxin B1 nan manje. .Dapre Òganizasyon Manje ak Agrikilti (FAO), nivo maksimòm aflatoksin B1 tolere atravè lemond yo te nan ranje 1-20 μg / kg nan manje, ak 5-50 μg / kg nan manje bèt dyetetik nan 2003.


Enstriksyon pou itilize

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Konsènan

Twous sa a ka itilize pou analiz kalitatif ak quantitative aflatoxin B1 nan lwil manjab, pistach, grenn sereyal, sòs soya, vinèg ak manje (bwit manje, materyèl pakèt melanje ak materyèl konsantre). analiz enstrimantal.

Pwodui sa a baze sou ELISA endirèk konpetitif, ki rapid, egzat ak sansib konpare ak analiz enstrimantal konvansyonèl yo.Li bezwen sèlman 45min nan yon sèl operasyon, ki ka konsiderableman redwi erè operasyon ak entansite travay.

Konpozan twous

• Mikrotiter plak prekouch ak antijèn, 96 pwi

• Solisyon Creole × 6bottle (1ml / boutèy)

0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb

• Anzim konjige 7ml…………………………………………………………………..………bouchon wouj

• Solisyon antikò 7ml................................................................. .................………bouchon vèt

• Substra A 7ml………………………………………………………….………………bouchon blan

• Substra B 7ml……………………………………………………………………….…………bouchon wouj

• Stop solisyon 7ml……….…….…………………………………………………………..………bouchon jòn

• 20×konsantre solisyon lave 40ml ………………………………………… bouchon transparan

• 2×konsantre solisyon ekstraksyon 50ml……………………………………………bouchon ble

Sansiblite, presizyon ak presizyon

Sansiblite:0.05ppb

Limit deteksyon

Echantiyon lwil manjab.................................................. .........................................................................0.1ppb

Pistach .................................................. .................................................. .......................0.2ppb

Sereyal................................................. .................................................. ......................0.05ppb

Presizyon

Echantiyon lwil manjab.................................................. .......................................................................80±15%

Pistach .................................................. .................................................. .....................80±15%

Sereyal................................................. .................................................. .....................80±15%

Presizyon

Varyasyon koyefisyan nan twous ELISA a se mwens pase 10%.

Kwa Pousantaj

Aflatoksin B1·················100%

Aflatoksin B2······················81 .3%

Aflatoksin G1······················62%

Aflatoksin G2·······················22.3%

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