khoom

Nitrofurans yog metabolized nyob rau hauv lub cev ceev heev, thiab lawv cov metabolites ua ke nrog cov ntaub so ntswg yuav muaj nyob rau lub sij hawm ntev, yog li kev soj ntsuam seem ntawm cov tshuaj no yuav nyob ntawm seb lawv cov metabolites, xws li furazolidone metabolite (AOZ), furaltadone metabolite (AMOZ). ), nitrofurantoin metabolite (AHD) thiab nitrofurazone metabolite (SEM).

Kwinbon Competitive Enzyme Immunoassay cov khoom siv, tseem hu ua Elisa cov khoom siv, yog cov cuab yeej bioassay raws li txoj cai ntawm Enzyme-Linked Immunosorbent Assay (ELISA). Nws qhov zoo yog feem ntau cuam tshuam nrog cov hauv qab no:

 (1) Ceev ceev: Feem ntau cov chaw sim txais LC-MS thiab LC-MS / MS txhawm rau ntes nitrofurazone metabolite. Txawm li cas los xij Kwinbon ELISA xeem, uas tshwj xeeb antibody ntawm SEM derivative yog qhov tseeb dua, rhiab, thiab yooj yim rau kev khiav lag luam. Lub sijhawm ntsuas ntawm cov khoom siv no tsuas yog 1.5h, uas yog qhov ua tau zoo kom tau txais cov txiaj ntsig. Qhov no yog qhov tseem ceeb rau kev kuaj mob sai thiab txo qis kev ua haujlwm.

(2) Qhov tseeb: Vim muaj qhov tshwj xeeb siab thiab rhiab heev ntawm cov khoom siv Kwinbon SEM Elisa, cov txiaj ntsig tau zoo heev nrog cov npoo qis ntawm qhov yuam kev. Qhov no ua rau nws tau siv dav hauv cov chaw kuaj mob thiab cov chaw tshawb fawb los pab cov chaw nuv ntses thiab cov khoom xa tawm hauv dej hauv kev kuaj mob thiab saib xyuas cov tshuaj tua tsiaj hauv SEM cov khoom hauv dej.

(3) High specification: Kwinbon SEM Elisa cov khoom siv muaj qhov tshwj xeeb thiab tuaj yeem kuaj tau cov tshuaj tiv thaiv tshwj xeeb. Qhov sib txawv ntawm SEM thiab nws cov metabolite yog 100%. Corss cov tshuaj tiv thaiv qhia tsawg dua 0.1% ntawm AOZ, AMOZ, AHD, CAP thiab lawv cov metabolites, Nws pab kom tsis txhob muaj qhov tsis zoo thiab tsis pom zoo.