Amid qhov tshwm sim hnyav zuj zus ntawm cov teeb meem kev nyab xeeb ntawm zaub mov, ib hom tshiab ntawm cov khoom siv kuaj raws li covEnzyme-Linked Immunosorbent Assay (ELISA)yog maj mam dhau los ua cov cuab yeej tseem ceeb hauv kev soj ntsuam kev nyab xeeb ntawm zaub mov. Nws tsis tsuas yog muab cov ntsiab lus meej thiab siv tau zoo rau kev saib xyuas cov zaub mov zoo tab sis kuj tsim cov kab kev tiv thaiv kom ruaj ntseg ntawm cov neeg siv khoom noj.
Lub hauv paus ntsiab lus ntawm ELISA cov khoom siv ntsuas yog nyob rau hauv kev siv cov tshuaj tiv thaiv tshwj xeeb ntawm cov tshuaj tiv thaiv antigen thiab cov tshuaj tiv thaiv kab mob kom txiav txim siab ntau npaum li cas cov ntsiab lus ntawm cov tshuaj hauv cov zaub mov los ntawm enzyme-catalyzed substrate xim kev loj hlob. Nws cov txheej txheem kev ua haujlwm yog qhov yooj yim thiab muaj qhov tshwj xeeb thiab rhiab heev, ua kom paub tseeb thiab ntsuas cov khoom tsis zoo hauv cov zaub mov, xws li aflatoxin, ochratoxin A, thiabT-2 co toxins.
Hais txog cov txheej txheem ua haujlwm tshwj xeeb, ELISA cov khoom siv kuaj feem ntau suav nrog cov kauj ruam hauv qab no:
1. Kev npaj ua qauv: Ua ntej, cov qauv zaub mov yuav tsum tau ua kom tsim nyog, xws li kev rho tawm thiab ua kom huv, kom tau txais cov qauv kev daws teeb meem uas tuaj yeem siv los kuaj xyuas.
2. Cov qauv ntxiv: Cov qauv ua tiav tau muab ntxiv rau hauv qhov dej uas tau teev tseg hauv ELISA phaj, nrog rau txhua qhov dej sib raug rau cov khoom siv los kuaj.
3. Incubation: ELISA phaj nrog cov qauv ntxiv yog incubated ntawm qhov kub thiab txias rau ib lub sij hawm kom cia tag nrho khi ntawm antigens thiab cov tshuaj tiv thaiv.
4. Ntxuav: Tom qab incubation, ntxuav cov tshuaj yog siv los tshem tawm unbound antigens los yog antibodies, txo cov kev cuam tshuam ntawm nonspecific binding.
5.Substrate ntxiv thiab kev loj hlob xim: Substrate tov yog ntxiv rau txhua qhov dej, thiab cov enzyme ntawm cov enzyme-labeled antibody catalyzes substrate los tsim cov xim, tsim cov khoom xim.
6. Kev Ntsuas: Tus nqi nqus ntawm cov khoom xim hauv txhua qhov dej yog ntsuas los ntawm kev siv cov cuab yeej xws li ELISA nyeem ntawv. Cov ntsiab lus ntawm cov tshuaj yuav tsum tau kuaj yog xam raws li tus qauv nkhaus.
Muaj ntau ntau daim ntawv thov ntawm ELISA cov khoom siv hauv kev kuaj khoom noj khoom haus. Piv txwv li, thaum lub sij hawm niaj hnub saib xyuas kev nyab xeeb ntawm zaub mov thiab kev soj ntsuam kuaj, cov tub ceev xwm tswj hwm kev lag luam tau siv cov khoom siv ELISA kom sai thiab raug kuaj pom ntau dhau ntawm aflatoxin B1 hauv cov txiv laum huab xeeb uas tsim los ntawm cov roj zeb. Cov kev nplua tsim nyog tau raug coj mus sai, tiv thaiv cov khoom tsis zoo los ntawm cov neeg siv khoom txaus ntshai.
Ntxiv mus, vim nws yooj yim ntawm kev ua haujlwm, raug, thiab kev ntseeg siab, ELISA cov khoom siv tau siv dav hauv kev ntsuas kev nyab xeeb ntawm ntau yam khoom noj xws li cov dej hauv dej, cov khoom noj nqaij, thiab cov khoom noj siv mis. Nws tsis tsuas yog txo lub sijhawm tshawb pom thiab txhim kho kev ua tau zoo tab sis kuj tseem muab kev txhawb nqa muaj zog rau cov tswj hwm kev tswj hwm kom ntxiv dag zog rau kev saib xyuas ntawm cov khw muag khoom noj.
Nrog kev nce qib ntawm kev siv thev naus laus zis thiab kev paub txog kev nyab xeeb ntawm cov zaub mov ntawm tib neeg, ELISA cov khoom siv kuaj yuav ua lub luag haujlwm tseem ceeb hauv kev soj ntsuam kev nyab xeeb khoom noj. Nyob rau hauv lub neej yav tom ntej, peb cia siab tias yuav tshwm sim tsis tu ncua ntawm kev siv technology tshiab, sib koom ua ke txhawb kev loj hlob ntawm kev lag luam kev nyab xeeb khoom noj khoom haus thiab muab kev ruaj ntseg ntxiv rau kev nyab xeeb ntawm cov neeg siv khoom noj khoom haus.
Post lub sij hawm: Dec-12-2024