Elisa Test Kit ntawm Ochratoxin A
Txog
Cov khoom siv no tuaj yeem siv rau hauv qhov ntau thiab qhov zoo ntawm ochratoxin A hauv kev pub.Nws yog ib yam khoom tshiab rau kev tshawb nrhiav tshuaj residue raws li ELISA thev naus laus zis, uas tsuas yog tus nqi 30min hauv txhua qhov kev ua haujlwm thiab tuaj yeem txo qis kev ua haujlwm tsis raug thiab ua haujlwm hnyav.Cov khoom siv no yog ua raws li kev sib tw tsis ncaj ncees ELISA thev naus laus zis.Lub qhov dej microtiter yog coated nrog coupling antigen.Ochratoxin A hauv cov qauv sib tw nrog cov antigen coated ntawm microtiter phaj rau lub ntibody ntxiv .Tom qab qhov sib ntxiv ntawm enzyme conjugate, TMB substrate yog siv los qhia cov xim.Kev nqus ntawm cov qauv tsis zoo cuam tshuam nrog o chratoxin A residue hauv nws, tom qab muab piv nrog Standard Curve, multiplied los ntawm dilution yam,Ochratoxin Ib qhov ntau hauv cov qauv tuaj yeem suav tau.
Cov khoom siv
• Microtiter phaj nrog 96 qhov dej coated nrog antigen
•Standard tov (6 fwj: 1ml / lub raj mis)
0 npi pb, 0.4ppb, 0.8 uappb, 1.6ppb, 3.2ppb, 6.4ppb
• Enzymesib txuas7ml…………………………………………………………………..………..…..liab cap
• Tshuaj Antibody10ml…………………………………………………………………...….… ntsuab cap
•Substrate solution A 7ml ………………………………………………………………………………
•SubstrateSolution B 7ml…………………………………………………………………..………………………… red cap
• Nres tshuaj 7ml ………………………………………………………………………….…………………… . . . . . . . . . . . . . .
• 20 × concentrated Ntxuav kua 40ml ………..……………………………………….…...... pob tshab cap
rhiab heev, raug thiab precision
Test Sensitivity: 0.4ppb
Kev kuaj pom txwv
Pub…………………………………………………….……………………………. 5 ppb
Qhov tseeb
Pub………………………………………………………………….90 ± 20%
Precision
Variation coefficient ntawm ELISA cov khoom siv tsawg dua 10%.
Hla tus nqi
Ochratoxin A……………………………………………………..………………..100%