khoom

Chloramphenicol yog ib qho tshuaj tua kab mob broad-spectrum, feem ntau siv rau hauv kev kho mob ntawm ntau yam kab mob ntawm cov tsiaj, thiab muaj inhibitory nyhuv ntawm ntau yam kab mob pathogenic.Teeb meem loj nrog chloramphenicol residues.Chloramphenicol muaj cov tshuaj lom thiab cov kev mob tshwm sim loj heev, uas tuaj yeem cuam tshuam lub hematopoietic kev ua haujlwm ntawm tib neeg cov pob txha pob txha, ua rau tib neeg aplastic anemia, granular leukocytosis, neonatal, ntxov ntxov grey syndrome thiab lwm yam kab mob, thiab tsawg concentration ntawm cov tshuaj residues kuj induce kab mob.Yog li ntawd, chloramphenicol residues nyob rau hauv cov khoom noj tsiaj ua rau muaj kev hem thawj loj rau tib neeg noj qab haus huv.Yog li, nws tau raug txwv lossis txwv tsis pub siv hauv EU thiab Asmeskas.

Kwinbon cov khoom siv no yog cov khoom siv tshiab raws li ELISA, uas yog ceev (tsuas yog 50min hauv ib qho kev ua haujlwm), yooj yim, raug thiab rhiab piv nrog cov cuab yeej ntsuas, thiab yog li nws tuaj yeem txo qis kev ua haujlwm yuam kev thiab ua haujlwm hnyav.

Kev sib tshuam

Chloramphenicol …………………………………………………… 100%

Chloramphenicol palmitate……………………………<0.1%

Thiamphenicol……………………………………………………..<0.1%

Florfenicol……………………………………………………<0.1%

Cetofenicol……………………………………………………..<0.1%

Cov khoom siv

Microtiter phaj coated nrog antigen, 96wells

Txuj kev daws teeb meem (6 × 1ml / lub raj mis)

0 ppb, 0.025 ppb, 0.075 ppb, 0.3 ppb, 1.2 ppb, 4.8 ppb

Spiking standard tov: (1ml / lub raj mis) ......100 ppb

Concentrated enzyme conjugate 1ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Enzyme conjugate diluent 10ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Solution A 7ml ................................................................................. . . . . . . . . . . . . . . . . . . . . .

Solution B 7ml………………………………………………………………… . . . . . . . . . . . .

Stop solution 7ml ................................................................................. ........ . . . . . . . . . . . . . . . . . . . .

20 × Concentrated ntxuav kua 40ml …………………………………………. pob tshab cap

2 × Concentrated extraction tov 50ml ................................................................. ...........blue cap

Cov txiaj ntsig

1 Feem pua ​​absorbance

Qhov nruab nrab qhov tseem ceeb ntawm absorbance qhov tseem ceeb tau txais rau cov qauv thiab cov qauv muab faib los ntawm tus nqi absorbance ntawm thawj tus qauv (zero standard) thiab muab faib ua 100%.Tus qauv xoom yog li ua kom sib npaug li 100% thiab cov txiaj ntsig absorbance tau hais hauv feem pua.

B —— nqus tus qauv (los yog qauv)

B₀ —— absorbance zero standard

2 Standard Curve

Txhawm rau kos tus qauv nkhaus: coj tus nqi nqus ntawm cov qauv raws li y-axis, semi logarithmic ntawm qhov concentration ntawm CAP cov qauv kev daws teeb meem (ppb) raws li x-axis.

Qhov CAP concentration ntawm txhua tus qauv (ppb), uas tuaj yeem nyeem los ntawm qhov ntsuas qhov nkhaus, yog muab faib los ntawm qhov sib thooj Dilution yam ntawm txhua tus qauv ua raws, thiab qhov tseeb concentration ntawm cov qauv tau txais.

Thov ceeb toom:

Rau kev txheeb xyuas cov ntaub ntawv ntawm ELISA cov khoom siv, software tshwj xeeb tau tsim, uas tuaj yeem txiav txim raws li kev thov.