Elisa Test Kit ntawm AMOZ
Txog
Cov khoom siv no tuaj yeem siv rau hauv qhov ntau thiab cov kev soj ntsuam zoo ntawm AMOZ residue hauv cov khoom hauv dej (ntses thiab cw), thiab lwm yam. Enzyme immunoassays, piv nrog cov txheej txheem chromatographic, qhia txog qhov muaj txiaj ntsig zoo txog kev nkag siab, kev tshawb nrhiav txwv, cov cuab yeej siv thiab lub sijhawm xav tau.
Cov khoom siv no yog tsim los ntes AMOZ raws li lub hauv paus ntsiab lus ntawm kev sib tw enzyme immunoassay.Lub qhov dej microtiter yog coated nrog ntes BSA txuas
antigen.AMOZ hauv cov qauv sib tw nrog cov antigen coated ntawm microtiter phaj rau cov tshuaj tiv thaiv ntxiv.Tom qab qhov sib ntxiv ntawm enzyme conjugate, chromogenic substrate yog siv thiab cov teeb liab yog ntsuas los ntawm spectrophotometer.Kev nqus yog inversely proportional rau AM OZ concentration hauv cov qauv.
Cov khoom siv
· Microtiter phaj nrog 96 qhov dej coated nrog antigen
· Cov kev daws teeb meem (6 lub raj mis)
0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb, 4.05 ppb
· Spiking standard tov: (1ml/lub raj mis) …………………………………………………….100 ppb
· Enzyme conjugate 1ml…………………………………………………………………..red cap
· Antibody solution 7ml ………………………………………………………………………………….
· Solution A 7ml ………………………………………………………………….………………… white cap
· Solution B 7ml ……………………………………………………………………………….…………..…… liab
· nres daws 7ml ………………………………………………………………….…………… daj cap
· 20 × concentrated ntxuav kua 40ml ……………………………………………… pob tshab cap
· 2 × concentrated extraction solution 50ml …………………………………………………….blue cap
· 2-Nitrobenzaldehyde 15.1mg …………………………………………………………………
rhiab heev, raug thiab precision
rhiab heev: 0,05p wb
Kev kuaj pom txwv
Cov khoom lag luam hauv dej (ntses thiab cw)………….……… 0.1ppb
Qhov tseeb
Cov khoom lag luam hauv dej (ntses thiab cw) …………..……… 95 ± 25%
Precision:CV ntawm cov khoom siv ELISA tsawg dua 10%.
Hla tus nqi
Furaltadone metabolite (AMOZ) ……………………………………….………… 100%
Furazolidone metabolite (AMOZ) ………………………………………..………………..<0.1%
Nitrofurantoin metabolite (AHD) ……………………………………….……………<0.1%
Nitrofurazone metabolite (SEM) ……………………………………………………..…<0.1%
Furaltadone………………………………………………………………….…….11.1%
Furazolidone ………………………………………………………………….. . . . . . < 0.1%
Nitrofurantoin…………………………………………………………..<1%
Nitrofurazone …………………………………………………….………………...<1%