Hoʻolālā ʻia kēia pahu ELISA e ʻike i nā quinolones e pili ana i ke kumu o ka immunoassay enzyme indirect-competitive. Ua uhi ʻia nā pūnāwai microtiter me ka antigen pili i ka BSA. Hoʻokūkū ʻo Quinolones i ka laʻana me ka antigen i uhi ʻia ma ka pā microtitre no ka antibody. Ma hope o ka hoʻohui ʻana o ka enzyme conjugate, hoʻohana ʻia ka substrate chromogenic a ana ʻia ka hōʻailona e ka spectrophotometer. Hoʻohālikelike ʻia ka absorption me ka ʻike quinolones i ka hāpana.