ʻO Elisa Test Kit o Ochratoxin A
E pili ana
Hiki ke hoʻohana ʻia kēia pahu i ka quantitative a qualitative analysis of ochratoxin A i ka hānai.He huahana hou ia no ka ʻike ʻana i ke koena o ka lāʻau e pili ana i ka ʻenehana ELISA, he 30 mau minuke wale nō ke kumukūʻai i kēlā me kēia hana a hiki ke hōʻemi nui i nā hewa hana a me ka ikaika o ka hana.Hoʻokumu ʻia kēia pahu i ka ʻenehana ELISA hoʻokūkū kūwaho.Hoʻopili ʻia nā pūnāwai microtiter me nā antigen hoʻohui.Hoʻokūkū ʻo Ochratoxin A i ka laʻana me ka antigen i uhi ʻia ma ka pā microtiter no ka ntibody i hoʻohui ʻia.Ma hope o ka hoʻohui ʻana i ka conjugate enzyme, hoʻohana ʻia ka substrate TMB e hōʻike i ke kala.Hoʻopili maikaʻi ʻole ka absorbance o ka hāpana i ke koena o chratoxin A i loko, ma hope o ka hoʻohālikelike ʻana me ka Curve Standard, i hoʻonui ʻia e nā mea dilution.Ochratoxin Hiki ke helu ʻia ka nui o ka hāpana.
Nā ʻāpana pahu
• ʻO ka pā Microtiter me nā pūnāwai 96 i uhi ʻia me ka antigen
•SʻO nā hāʻina tandard (6 ʻōmole: 1ml / ʻōmole)
0ppb, 0.4ppb, 0.8ppb, 1.6ppb, 3.2ppb, 6.4ppb
• Enzymeconjugate7ml……………………………………………………..……..…..pāpale ʻulaʻula
• Hoʻonā antibody10ml………………………………………………………………...….… pāpale ʻōmaʻomaʻo
•Pāpala sʻoluʻolu A 7ml……………………………………………………………………………………
•PāpaʻaPane B 7ml……………………………………………………..…………………… ʻulaʻula
• Hoʻopau i ka hoʻonā 7ml ………………………………………………………………….…………………… pāpale melemele
• 20×ka hoʻoheheʻe holoi holoi 40ml………..………………………………….…...… pāpale alohilohi
ʻIke, pololei a me ka pololei
Hoʻomaʻamaʻa hoʻāʻo: 0.4ppb
palena ʻike
Hānai…………………………………………………….………..……………….5ppb
Ka pololei
Hānai…………………………………………………….…………………..90±20%
pololei
ʻOi aku ka liʻiliʻi o ka helu hoʻololi o ka pahu ELISA ma mua o 10%.
Uku Kea
Ochratoxin A………………………………………………..……………..100%