Kiko Ho'āʻo Elisa o AOZ
E pili ana
Hiki ke hoʻohana ʻia kēia kit i ka quantitative a qualitative analysis of AOZ koena i loko o nā ʻiʻo holoholona (moa, pipi, puaʻa, etc.), ka waiū, ka meli a me nā hua.
Pono ke kālailai ʻana o ke koena o nā lāʻau nitrofuran ma muli o ka ʻike ʻana i nā metabolites paʻa i ka ʻiʻo o nā lāʻau makua nitrofuran, ʻo ia hoʻi me Furazolidone metabolite (AOZ), Furaltadone metabolite (AMOZ), Nitrofurantoin metabolite (AHD) a me Nitrofurazone metabolite (SEM).
Ke hoʻohālikelike ʻia me nā ʻano chromatographic, hōʻike kā mākou kit i nā pōmaikaʻi nui e pili ana i ka naʻau, ka palena ʻike, nā mea ʻenehana a me nā koi manawa.
Nā ʻāpana pahu
• ʻO ka pā Microtiter i uhi ʻia me ka antigen, 96 pūnāwai
• Nā hāʻina maʻamau (6 ʻōmole, 1ml/ʻōmole)
0ppb,0.025ppb,0.075ppb,0.225ppb,0.675ppb,2.025ppb
• Spiking standard control : (1ml/bottle)...................................................….100ppb
• Enzyme conjugate concentrate 1.5ml.................................................…..pale ʻulaʻula
• 0.8ml ka hoʻonā antibody …………………………………...… pāpale ʻōmaʻomaʻo
• Pāpā A 7ml……………………...........................................................pale keʻokeʻo
• Pāpala B7ml………………………………………….........................................……..palena ʻulaʻula
• Hoʻopau i ka solution 7ml…………………………………………………….…… pāpale melemele
• 20×holoi holoi huikau 40ml ……………………………………
• 2×ka hoʻoheheʻe ʻia 60ml……………………..………….palena uliuli
• 2-Nitrobenzaldehyde 15.1mg………………………………………………
ʻIke, pololei a me ka pololei
ʻO ka ʻike: 0.025ppb
palena ʻike……………………………………………0.1ppb
pololei:
ʻO ka ʻiʻo holoholona (ka ʻiʻo a me ke akepaʻa)………………………… 75±15%
Meli……………………………………………………..90±20%
Hua……………………………………………..…90±20%
Waiu………………………………………………………..…90±10%
pololei:ʻO ka CV o ka pahu ELISA he emi iho ma mua o 10%.
Uku Kea
Furazolidone metabolite (AOZ)…………………………………………………..100%
Furaltadone metabolite (AMOZ)……………………………………………………<0.1%
Nitrofurantoin metabolite (AHD)…………………………………………<0.1%
Nitrofurazone metabolite (SEM)…………………………………………...<0.1%
Furazolidone………………………………………………………………..…16.3%
Furaltadone……………………………………………………………………<1%
Nitrofurantoin……………………………………………………………….…<1%
Nitrofurazone……………………………………………………………………..<1%