An tsara wannan kit ɗin ELISA don gano quinolones bisa ka'idar immunoassay enzyme mai gasa kai tsaye. An lulluɓe rijiyoyin microtiter tare da kama antigen mai alaƙa da BSA. Quinolones a cikin samfurin suna gasa tare da antigen da aka lulluɓe akan farantin microtitre don maganin rigakafi. Bayan da aka ƙara enzyme conjugate, ana amfani da substrate na chromogenic kuma ana auna siginar ta spectrophotometer. Abun sha ya bambanta da daidaituwar quinolones a cikin samfurin.