Kit deuchainn Elisa de Aflatoxin B1
Bidh dòsan mòra de aflatoxins a’ leantainn gu puinnseanachadh gann (aflatoxicosis) a dh’ fhaodadh a bhith na chunnart beatha, mar as trice tro mhilleadh air an grùthan.
Tha aflatoxin B1 na aflatoxin air a dhèanamh le Aspergillus flavus agus A. parasiticus.Tha e na carcinogen gu math cumhachdach.Tha an comas carcinogenic seo ag atharrachadh thar ghnèithean le cuid, leithid radain is muncaidhean, a rèir coltais tòrr nas so-leònte na cuid eile.Tha aflatoxin B1 na thruailleadh cumanta ann am measgachadh de bhiadhan a’ toirt a-steach cnòthan-cnòthan, min-chotain, arbhar agus gràinean eile;a bharrachd air biadh bheathaichean.Thathas den bheachd gur e Aflatoxin B1 an aflatoxin as puinnseanta agus tha e gu mòr an sàs ann an carcinoma hepatocellular (HCC) ann an daoine.Chaidh grunn dhòighean samplachaidh is anailis a’ toirt a-steach cromatagrafaidheachd tana-fhilleadh (TLC), cromatagrafaidheachd lionn àrd-choileanadh (HPLC), mòr-speactrometry, agus assay immunosorbent ceangailte le enzyme (ELISA), am measg feadhainn eile, a chleachdadh gus deuchainn a dhèanamh airson truailleadh aflatoxin B1 ann am biadhan. .A rèir Buidheann Bidhe is Àiteachais (FAO), chaidh aithris gu robh na h-ìrean ceadaichte as àirde air feadh an t-saoghail de aflatoxin B1 anns an raon de 1–20 μg/kg ann am biadh, agus 5–50 μg/kg ann am biadhadh crodh daithead ann an 2003.
Mion-fhiosrachadh
Kit Deuchainn 1.Elisa airson Aflatoxin B1
2.Cait.KA07202H-96tobraichean
3. Kit co-phàirtean
● Microtiter plateprecoated le antigen, 96 tobraichean
● Fuasgladh Coitcheann × 6botal (1ml / botal)
0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb
● Enzyme conjugate 7ml ………………………………………………………..………… ceap dearg
● Fuasgladh antibody7ml ...................................................... ...................................... bonaid uaine
● Fo-strat A 7ml………………………………………………………….…………...…bonn geal
● Fo-strat B 7ml……………….………………………………………………………………………….………… ceap dearg
● Fuasgladh stad 7ml ………….…….…………………………………………..………caip buidhe
● Fuasgladh nighe cuimseach 20 × 40ml ………………………………………… ceap follaiseach
● Fuasgladh às-tharraing cuimseach 2 × 50ml……………………………………………… ceap gorm
4.Sensitivity, mionaideachd agus mionaideachd
Cugallachd: 0.05ppb
5.Detection crìoch
Sampall ola a ghabhas ithe ........................................................... ...................................................... ...................... 0.1ppb
Peanut................................................... ...................................................... ...................... 0.2ppb
Arbhar................................................... ...................................................... ......................0.05ppb
Cruinneas
Sampall ola a ghabhas ithe ........................................................... ...................................................... ......................80±15%
Peanut................................................... ...................................................... ......................80±15%
Arbhar................................................... ...................................................... ......................80±15%
Cruinneas:Tha co-èifeachd eadar-dhealachaidh an uidheamachd ELISA nas lugha na 10%.
6.Cross Ìre
Aflatocsin B1················ 100%
Aflatocsin B2····················81 .3%
Aflatocsin G1···················· 62%
Aflatocsin G2···················· 22.3%