ELisa Test Kit fan AOZ
Oer
Dizze kit kin brûkt wurde yn kwantitative en kwalitative analyze fan AOZ-residu yn dierlike weefsels (kip, fee, pig, ensfh), molke, huning en aaien.
De analyze fan residuele nitrofuran-medisinen moat basearre wurde op 'e deteksje fan' e weefsel-bûne metabolites fan 'e nitrofuran-âldermedisinen, dy't Furazolidone-metabolite (AOZ), Furaltadon-metabolite (AMOZ), Nitrofurantoin-metabolite (AHD) en Nitrofurazone-metabolite (SEM) omfetsje.
Yn ferliking mei chromatografyske metoaden toant ús kit grutte foardielen oangeande gefoelichheid, deteksjelimyt, technyske apparatuer en tiideasken.
Kit Components
• Microtiter plaat bedekt mei antigen, 96 wells
• Standert oplossingen (6 flessen, 1 ml / flesse)
0ppb,0.025ppb,0.075ppb,0.225ppb,0.675ppb,2.025ppb
• Spiking standert kontrôle: (1ml / flesse)................................................….100 ppb
• Enzym konjugat konsintraat 1.5ml ......................................................…….reade pet
• Antibody oplossing konsintrearre 0.8ml…………………………………......griene pet
• Substraat A 7ml……….................................................................……………………..witte pet
• Substraat B7ml………......................................................................……….read pet
• Stop oplossing 7ml……………………………………………………….………giele pet
• 20 × konsintrearre wask oplossing 40ml ……………………….……transparante kap
• 2×konsintrearre ekstraksjeoplossing 60ml…………………..………………… blauwe pet
• 2-Nitrobenzaldehyde 15.1mg…………………………………………………………swarte pet
Gefoelichheid, krektens en krektens
Gefoelichheid: 0.025 ppb
Detection limyt…………………………………………………0.1ppb
Krektens:
Dierweefsel (spier en lever)…………………75±15%
Honey………………………………………………………………..90±20%
Ei………………………………………..…………………..…90±20%
Molke…………………………………………………………………..…90±10%
Krektens:CV fan 'e ELISA-kit is minder dan 10%.
Cross Rate
Furazolidon metabolite (AOZ) …………………………………………………..100%
Furaltadone metabolite (AMOZ)…………………………………………………<0.1%
Nitrofurantoin metabolite (AHD) ………………………………………… <0.1%
Nitrofurazon metabolite (SEM)…………………………………………………………………<0.1%
Furazolidone………………………………………………………………………..…16,3%
Furaltadone………………………………………………………………………..<1%
Nitrofurantoin……………………………………………………………….…….…<1%
Nitrofurazon…………………………………………………………………………..…<1%